Biolegend, 671301, Purified anti-UFD1 Antibody, 25μg

UFD1 forms a ternary complex with Nuclear protein localization protein 4 homolog (NPLOC4) and valosin-containing Protein (VCP), and this complex plays an essential role in detecting and binding ubiquinated proteins. The complex also aids in the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear membrane after mitosis occurs.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant human anti-UFD1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1 µg per ml. For immunocytochemistry, a concentration of 1 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When stained for ICC, the methanol permeabilization method proved more effective than the Triton X-100 permeabilization method in localizing expression in the endoplasmic reticulum.
RRID: AB_2814530 (BioLegend Cat. No. 671301) AB_2814531 (BioLegend Cat. No. 671302)
Structure: UFD1 is a 307 amino-acid protein with a predicted molecular weight of 38 kD.
Distribution: Localized to endoplasmic reticulum; ubiquitously expressed
Function: Ubiquitinated protein degradation; mitotic spindle degradation; nuclear envelope formation
Interaction: NPLOC4; VCP
Cell Type: Dendritic cells
Biology Area: Cell Biology, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Antigen References: Pizzuti A, et al. 1997. Hum. Mol. Genet. 6(2):259-65. Botta A, et al. 2001. Gene. 275(1):39-46. Hao Q, et al. 2015. EMBO. J. 34(23):2903-20. Chen M, et al. 2011. Proc. Natl. Acad. Sci. USA. 108(22):9119-24.
Gene ID: 7353
UniProt: View information about UFD1 on UniProt.org
Clone: W18140A
Regulatory Status: RUO
Other Names: Ubiquitin Recognition Factor in ER-Associated Degradation 1, UB Fusion Protein 1, UFD1L, Ubiquitin Fusion Degradation Protein 1
Isotype: Rat IgG2a, κ