Biolegend, 671402, Purified anti-HYOU1 Antibody, 100μg

HYOU1 belongs to the heat shock protein 70 family. The protein encoded by this gene is involved in stress-dependent induction. As a result, under low-oxygen conditions, HYOU1 accumulates in the endoplasmic reticulum. HYOU1 also acts as a nucleotide exchange factor (NEF) that recognizes other proteins within the ER and participates in protein folding.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human HYOU1 protein corresponding to amino acids 750-999.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg per ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When stained for ICC, methanol permeabilization proved more effective than Triton-X 100 permeabilization in localizing expression to the endoplasmic reticulum.
RRID: AB_2814532 (BioLegend Cat. No. 671401) AB_2814533 (BioLegend Cat. No. 671402)
Structure: HYOU1 is a 999 amino-acid protein with a predicted molecular weight of 110 kD.
Distribution: Localized to the ER; Ubiquitously expressed
Function: Protein folding & secretion; stress-dependent induction
Molecular Family: Heat Shock Proteins, Organelle Markers
Antigen References: Ozawa K, et al. 1999. J. Biol. Chem. 274(10):6397-404. Ikeda J, et al. 1997. Biochem. Biophys. Res. Commun. 230(1):94-9. Takeuchi S. 2006. Protein J. 25(7-8):517-28. Kaneda S, et al. 2000. J Biochem. 128(3):529-38.
Gene ID: 10525
UniProt: View information about HYOU1 on UniProt.org
Clone: W18144A
Regulatory Status: RUO
Other Names: Hypoxia Up-Regulated 1, GRP-170, ORP-150, 170 kD Glucose-Regulated Protein 150 kD Oxygen-Regulated Protein
Isotype: Rat IgG2a, κ