Biolegend, 672952, Purified anti-GDF3 Antibody, 100μg

GDF3 (Growth/Differentiation Factor 3) is a member of the transforming growth factor-beta (TGF-β) superfamily, and is involved in regulating stem cell differentiation, tissue patterning, and embryonic development. GDF3 is particularly important in the development of the early embryo, influencing mesoderm and ectoderm formation, and left-right axis patterning. In stem cells GDF3 has been shown to modulate pluripotency, with its expression being critical for maintaining the undifferentiated state. GDF3 also interacts with other signaling pathways, including those of BMPs (bone morphogenetic proteins), to fine-tune cellular processes such as bone formation. In adults, GDF3 is involved in wound healing, the inflammatory response, and skeletal muscle remodeling.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human GDF3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). This product is not recommended for use in Western Blot (WB), immunocytochemistry (ICC) or intracellular flow cytometry (ICFC).
RRID: AB_3699090 (BioLegend Cat. No. 672951) AB_3699090 (BioLegend Cat. No. 672952)
Structure: GDF3 is a 364 amino acid protein with a predicted molecular weight of 41.4 kD.
Distribution: Cytoplasmic, secreted
Function: Growth factor
Ligand/Receptor: TGFBR1, TGFBR2, ALK7
Biology Area: Cell Biology, Stem Cells
Molecular Family: Growth Factors
Antigen References: Levine AJ, Brivanlou AH. 2006. Cell Cycle. 5:1069-73. Levine AJ, Brivanlou AH. 2006. Development. 133:209-16. Bisgrove BW, et al. 2017. Elife. 6:e28534. Chen C, et al. 2006. Development. 133:319-29.
Gene ID: 9573
UniProt: View information about GDF3 on UniProt.org
Clone: A21102G
Regulatory Status: RUO
Other Names: Growth/differentiation factor 3, GDF-3
Isotype: Mouse IgG2b, κ