Biolegend, 673251, Purified anti-SNAIL Antibody, 25μg

SNAIL is a zinc finger transcriptional repressor that is involved in cell movement, adhesion, and survival. SNAIL plays a pivotal role in the epithelial to mesenchymal transition (EMT) during embryonic development. SNAIL represses the expression of E-cadherin in epithelial cells leading to the disassembly of cell-cell junctions, loss of cell polarity, and increased migration. In pathological contexts, SNAIL can promote tumor progression and metastasis by allowing cancer cells to invade surrounding tissues.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human SNAIL protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody in immunocytochemistry (ICC), we recommend fixation/permeabilization with PFA 2% and Triton X-100 0.5%. We do not recommend PFA/methanol or methanol alone.
RRID: AB_3683495 (BioLegend Cat. No. 673251) AB_3683495 (BioLegend Cat. No. 673252)
Structure: SNAIL is a 264 amino acid protein with a molecular weight of approximately 29 kDa.
Distribution: SNAIL is primarily located in the nucleus of the cell but can be transported to the cytoplasm upon phosphorylation.
Function: EMT, embryonic development, cell migration
Interaction: FBXL14, GSK3B, BTRC, WTIP, LIMD1 LOXL2 and LOXL3, CSNK2A1, EGR1, LATS2, KPNB1, TNPO1, KPNA1, KPNA4, IPO7, PARP1, KDM1A, p53, NOTCH1, MDM2, CSNK1E, and PAK1
Antigen References: Dong B, et al. 2021. Int J Mol Sci. 22:11062. Olmeda D, et al. 2007. Cancer Res. 67:11721. Baulida J, et al. 2019. J Clin Med. 8: 757. Suzuki T, et al. 2023. J Cancer Metastasis Treat. 9:25. Sun JX, et al. 2018. Angiogenesis. 21:635.
Gene ID: 6615
UniProt: View information about SNAIL on UniProt.org
Clone: W22252A
Regulatory Status: RUO
Other Names: SNAI1, Snail Family Transcriptional Repressor, SNAH, SLUGH2, SNAIL, SNA, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1
Isotype: Rat IgG2b, κ