Biolegend, 673451, Purified anti-S100A8 Antibody, 25μg

S100A8 is a member of the S100/calgranulin family of proteins containing 2EF-hand calcium-binding motifs. S100A8 is mainly expressed in cells of the myeloid lineage and is present in circulating neutrophils and monocytes. S100A8 expression is also transiently induced in keratinocytes after epidermal injury and in psoriasis where pro-inflammatory cytokines induce its expression. S100A8 is mostly cytoplasmic, but can be translocated to plasma membrane or released into the extracellular environment upon cellular activation. S100A8 preferentially interacts with S100A9 to form a heterodimer S100A8/A9, also known as calprotectin. S100A8/A9 functions as a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. The extracellular functions of S100A8/A9 also include the recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. S100A8/A9 displays apoptosis-inducing activity against various tumor cells and also has antimicrobial activity by depriving bacterial pathogens of essential trace metals such as Zn2+ and Mn2+.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human S100A8
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: Fixation buffer (Cat. No. 420801) followed by 100% ice-cold methanol 100% ice-cold methanol only
RRID: AB_3675191 (BioLegend Cat. No. 673451) AB_3675191 (BioLegend Cat. No. 673452)
Structure: Homodimer
Distribution: Neutrophils, Monocytes, Keratinocytes
Function: Pro-inflammatory molecule
Interaction: Neutrophils, Monocytes
Ligand/Receptor: Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (AGER)
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family: Heat Shock Proteins
Antigen References: Bhardwaj RS, et al. 1992 Eur. J. Immunol. 22:1891. Hunter MJ, et al. 1998 J. Biol. Chem. 273:12427. Hofmann MA, et al. 1999 Cell 97:889-901. Cross SS, et al. 2005 Histopathology 46:256. Zenz R, et al. 2005 Nature 437:369. Hermani A, et al. 2006 Exp. Cell Res. 312:184. Vogl T, et al. 2007 Nature Med. 13:1042. Benedyk M, et al. 2007 J. Invest. Dermatol. 127:2001. Corbin BD, et al. 2008 Science 319:962. Gebhardt C, et al. 2008 J. Exp. Med. 205:275. Loser K, et al. 2010 Nat Med. 16:713-17. Kang JH, et al. 2015 Immunology 144:79-90.
Gene ID: 6279
UniProt: View information about S100A8 on UniProt.org
Clone: A22124D
Regulatory Status: RUO
Other Names: S100 Calcium-binding protein A8, Cystic Fibrosis Antigen (CFAG), Calgranulin A (CAGA, CGLA), Myeloid-related protein 8 (MRP8), S10A8
Isotype: Mouse IgG2b, λ