Biolegend, 673551, Purified anti-TAZ (WWTR1) Antibody, 25μg

TAZ, also known as WWTR1, is a critical regulator in various cellular processes including cell proliferation, differentiation, and organ development. It is a key component of the Hippo signaling pathway, which controls organ size by regulating cell growth and apoptosis. TAZ functions primarily as a transcriptional co-activator, partnering with transcription factors such as TEAD to promote the expression of genes involved in cell cycle progression and survival. Additionally, it plays a role in maintaining stem cell pluripotency and promoting epithelial-mesenchymal transition (EMT), a process important in development and cancer metastasis. Aberrations in TAZ activity are linked to various cancers, highlighting its significance in both normal physiology and disease pathology. TAZ should not be confused with tafazzin, which was previously also known as Taz/Taz1.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the N-terminus of human TAZ (WWTR1)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody (clone W22256A) in immunocytochemistry (ICC) we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol. Permeabilization with 100% ice-cold methanol results in a dimmer signal compared to 0.5% Triton-X. Fixation/permeabilization with 100% ice-cold methanol alone is not recommended. For use of this antibody (clone W23013A) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using fixation buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401). For use of this antibody (clone W23013A) in immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703). Antigen retrieval with Sodium Citrate H.I.E.R. (Cat. No. 928502) is not recommended.
RRID: AB_3675192 (BioLegend Cat. No. 673551) AB_3675192 (BioLegend Cat. No. 673552)
Structure: TAZ is a 400 amino acid protein with a predicted molecular weight of 44.1 kD.
Distribution: Predominantly nucleus, with diffuse localization in the cytoplasm at high expression levels
Function: Transcription factor, transcriptional co-activator
Interaction: TEA domain family members (TEAD1/2/3/4), PAX3, RUNX1/2, YAP1, AP1, SMAD2/3/4
Biology Area: Cell Biology, Cell Proliferation and Viability, Stem Cells, Transcription Factors
Antigen References: Piccolo S, et al. 2014. Physiol. Rev. 94:1287-312. Moya I, Halder G. 2019. Nat. Rev. Mol. Cell Biol. 20:211-226. Pocaterra A, et al. 2020. J. Cell Sci. 133:jcs230425. Wei Y, et al. 2023. MedComm. 4:e340.
Gene ID: 25937
UniProt: View information about TAZ on UniProt.org
Clone: W22256A
Regulatory Status: RUO
Other Names: Transcriptional coactivator with PDZ-binding motif (TAZ), WW domain-containing transription regulator protein 1 (WWTR1)
Isotype: Rat IgG2a, κ