Biolegend, 673902, Purified anti-NLRP2 Antibody, 100μg

NLRP2, also known as NALP2 or PAN1, is a member of the pyrin domain-containing NLR protein (NLRP) family. The NLR proteins interact with ASC via the conserved pyrin domain, which results in the activation of caspase-1 and maturation of the pro-inflammatory cytokines IL-1β and IL-18. NLRP2 is also involved in the formation of the inflammasome and caspase-1 activation. NLRP2 negatively regulates NFκB activation, which is induced by TNF-α, through inhibiting NFKBIA degradation. In addition, NLRP2 is one of the maternal effect genes and plays a crucial role in embryonic development. In mouse embryos, knockdown or mutation of NLRP2 leads to developmental defections. Truncation of the NLRP2 gene is reported to associate with an overgrowth discorder called Beckwith-Wiedemann Syndrome.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial NLRP2 recombinant protein (60-192 aa) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted at 4°C
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2564486 (BioLegend Cat. No. 673902)
Structure: 1062 amino acids with a predicted molecular weight of 120.5 kD; contains a DAPIN domain, NACT domain, and eight LRR repeats.
Distribution: Cytoplasm
Function: NLRP2 is a component of inflammasome that is involved in the activation of caspase-1 and the inhibition of NK&κB signaling.
Interaction: NLRP2 interacts wtih IKBKB, IKBKG, CHUK, and MEFV.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Peng H, et al. 2012. PLoS One 7:e30344. 2. Lupfer C, et al. 2013. Front Immunol. 4:285. 3. Minkiewicz J, et al. 2013. Glia. 61:1113. 4. Huang JY, et al. 2013. Hum. Reprod. 28:1127. 5. Fontalba A, et al. 2007. J. Immunol. 179:8519. 6. Bruey JM, et al. 2004. J. Biol. Chem. 50:51897.
Gene ID: 55655
UniProt: View information about NLRP2 on UniProt.org
Clone: 8F10B51
Regulatory Status: RUO
Other Names: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1 (NBS-1), NBS1, PYRIN domain and NACHT domain-containing protein 1 (PAN1), PYRIN-containing APAF1-like protein 2 (PYPAF2)
Isotype: Mouse IgG1, κ