Biolegend, 674151, Purified anti-SIX3 Antibody, 25μg

The SIX3 protein is a transcription factor that plays a crucial role in the development of the forebrain and eyes. It functions by binding to specific DNA sequences, regulating the expression of target genes involved in these developmental processes through both repression and activation. During human forebrain development, SIX3 represses WNT1 expression to ensure proper patterning. Mutations in the SIX3 gene are associated with holoprosencephaly type 2 (HPE2), a severe congenital brain malformation characterized by incomplete separation of the forebrain. Additionally, SIX3 mutations have been linked to schizencephaly, a rare brain disorder that results in abnormal clefts or slits in the cerebral hemispheres.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of Human SIX3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401). For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using PFA-Methanol or PFA-Triton X-100. We do not recommend Methanol only fixation/permeabilization
RRID: AB_3699092 (BioLegend Cat. No. 674151) AB_3699092 (BioLegend Cat. No. 674152)
Structure: The SIX3 protein consists of 332 amino acids and has a molecular weight of approximately 35 kDa.
Distribution: Nuclear
Function: • Acts as a transcription factor, binding to specific DNA sequences to regulate gene expression. • Plays a critical role in the development of the forebrain and eyes. • Involved in the formation of the optic vesicles and the anterior-posterior patterning of the diencephalon. • Essential for proper lens development and the formation of the proximodistal axis of the optic cup.
Interaction: EYA4, MTA1, TLE1, TLE5
Cell Type: Neural Stem Cells, Neurons
Biology Area: Cell Biology, Neuroscience, Stem Cells, Transcription Factors
Antigen References: Ferrena A, et al. 2024. PLOS One. 19:e0308839 Song X, et al. 2021. Neurosci Bull. 37:985 Yu Z, et al. 2017. J Hematol Oncol. 10:115 Geng X, et al. 2016. Development. 143:4462
Gene ID: 6496
UniProt: View information about SIX3 on UniProt.org
Clone: W23154C
Regulatory Status: RUO
Other Names: Homeobox protein SIX3, HPE2, Sine oculis homebox-like protein 3, Sine oculis homeobox homolog 3
Isotype: Rat IgG2a, κ