Biolegend, 674201, Purified anti-Nanog Antibody, 25μg

Nanog is a homeodomain-containing transcription factor that is essential for early embryonic development. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer cell lines, maintaining the undifferentiated status of the cells and is downregulated upon differentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineage differentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 and SOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog is regulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in several types of tumor cells. The expression level of Nanog in cancer cells has been shown to be correlated with the malignancy of cancers.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length NANOG recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at 4°C
Application: WB - Quality tested ICFC, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml (1:200-1:1000 dilution). For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Houweling PJ, et al. 2021. Stem Cell Research. 54:102429. PubMed Bozaoglu K, et al. 2020. Stem Cell Res. 49:102013. PubMed
RRID: AB_2564573 (BioLegend Cat. No. 674201) AB_2564574 (BioLegend Cat. No. 674202)
Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding.
Interaction: Interacts with ZFP281, SMAD1, and SALL4.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587. 2. Villasante A, et al. 2011. Cell Cycle 10:1488. 3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222. 4. Pan G, et al. 2007. Cell Res. 17:42. 5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093. 6. Silva J, et al. 2009. Cell 138:722.
Gene ID: 79923
UniProt: View information about Nanog on UniProt.org
Clone: 16H3A48
Regulatory Status: RUO
Other Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype: Mouse IgG1, κ