Biolegend, 674802, Purified anti-Apo E Antibody, 100μg

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length mouse Apo-E recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Moon HJ, et al. 2022. Neurobiol Dis. 164:105631. PubMed Jeong A, et al. 2021. Acta Neuropathol Commun. 9:129. PubMed
RRID: AB_2565426 (BioLegend Cat. No. 674802)
Structure: 293 amino acids, predicted molecular weight of 34 kD (mature form), and contains one LDL receptor binding domain and two heparin binding regions.
Function: Apo-E is a component of serum chylomicron, VLDL, and HDL particles, and mediates lipid metabolism and memory formation.
Interaction: SET, KCNMA1, SAA, and PRNP.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Antigen References: 1. Hayes EM, et al. 2014. Front Immunol. 5:537. 2. Kim J, et al. 2014. Mol. Cells 37:767. 3. Verghese PB, et al. 2013. Proc. Natl. Acad. Sci. USA 110:E1807. 4. Mahley RW, et al. 2012. Neuron 76:871. 5. Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312. 6. Zhang HL, et al. 2010. Clin. Dev. Immunol. 2010:186813.
Gene ID: 11816
UniProt: View information about Apo E on UniProt.org
Clone: 3B3C32
Regulatory Status: RUO
Other Names: Apolipoprotein E, ApoE
Isotype: Rat IgG2a, κ