Biolegend, 675302, Purified anti-DDX1 Antibody, 100μg

DDX1, also known as DEAD box protein retinoblastoma, is a member of the DEAD (Asp-Glu-Ala-Asp)-box RNA helicase family. DDX1 possesses RNA nuclease, binding, unwinding, and ATPase activities, and is thus involved in RNA metabolism, pre-mRNA splicing, translation initiation, and ribosome biogenesis. DDX1 interacts with the Rev protein of HIV-1 and has been shown to be crucial in the viral RNA replication. DDX1 was found to locate to the area of DNA damage induced by ionizing radiation and is implicated to participate in double-strand DNA break repair. DDX1 also acts as a transcriptional coactivator to facilitate NF-κB mediated gene activation. Elevated expression of DDX1 was observed in a number of tumors such as retinoblastoma, neuroblastoma, and testicular carcinoma. The accumulation of DDX1 is associated with cancer metastasis and poor survival.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human DDX1 recombinant protein (1-107 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2565509 (BioLegend Cat. No. 675302)
Structure: 740 amino acids with a predicted molecular weight of 82 kD. It contains a DEAD box motif and an ATP-binding helicase domain.
Distribution: Nucleus and cytoplasm.
Function: DDX1 is an ATP-dependent RNA helicase and possesses RNA nuclease activity and ATPase activity. DDX1 has been shown to participate in mRNA processing, DNA repair, and transcriptional activation.
Interaction: PHF5A, MBNL1, CSTF2, HNRNPK, ATM, and NFκB-p65.
Biology Area: Cell Biology, DNA Repair/Replication, Transcription Factors
Antigen References: 1. Lin MH, et al. 2014. Retrovirology 11:121. 2. Han C, et al. 2014. Cell Rep. 8:1447. 3. Edgcomb SP, et al. 2012. J. Mol. Biol. 415:61. 4. Taunk NK, et al. 2012. Cancer 118:888. 5. Xu L, et al. 2010. J. Virol. 84:8571. 6. Godbout R, et al. 2007. Future Oncol. 3:575.
Gene ID: 1653
UniProt: View information about DDX1 on UniProt.org
Clone: 9F12B04
Regulatory Status: RUO
Other Names: ATP-dependent RNA helicase DDX1, DEAD box protein retinoblastoma (DBP-RB)
Isotype: Mouse IgG1, κ