Biolegend, 676802, Purified anti-HDAC4 Antibody, 100μg

HDAC4 is a member of the class II histone deacetylase family and is similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 has been shown to play a crucial role in skeletal formation. HDAC4 prevents chondrocyte hypertrophy through inhibiting Runx2 activity during endochondral bone formation. The HDAC4 deficient mice show chondrocyte hypertrophy and premature ossification of developing bones. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human HDAC4 recombinant protein (529 - 651 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 14A9C47 weakly cross-reacts with mouse HDAC4.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).In some experimental systems a non-specific band may be observed at ~75kD.
RRID: AB_2861052 (BioLegend Cat. No. 676801) AB_2565722 (BioLegend Cat. No. 676802)
Structure: 1084 amino acids with a predicted molecular weight of 119 kD. Contains a histone deacetylase region and a N-terminal homodimerization domain.
Distribution: Cytoplasm and nucleus.
Interaction: CDKN2A forms a homodimer and interacts with HDAC7, MEF2C, BTBD14B, AHRR, NR2C1, adn 14-3-3.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References: 1. Tong JK, et al. 1998. Nature 395:917. 2. Grunstein M. 1997. Nature 389:349. 3. Wolffe AP. 1996. Science 272:371. 4. Lee DY, et al. 1993. Cell 72:73. 5. Wang AH, et al. 2001. Mol. Cell Biol. 21:5992. 6. Bertos NR, et al. 1999. Mol. Cell Biol. 11:7816.
Gene ID: 9759
UniProt: View information about HDAC4 on UniProt.org
Clone: 14A9C47
Regulatory Status: RUO
Other Names: Histone deacetylase 4, HD4
Isotype: Mouse IgG2b, κ