Biolegend, 676902, Purified anti-IL-27 p28 Antibody, 100μg

IL-27 is a heterodimeric cytokine that is composed of two subunits (p28 and EBI3). It plays a critical role in T-cell proliferation and differentiation. It also drives rapid clonal expansion of naive but not memory CD4 T cells. IL-27 is a potent inhibitor of Th17 cell development and IL-17 production. IL-27 subunit p28 alone is able to inhibit the production of IL-17 by CD4 and CD8 T cells. Furthermore, IL-27 plays an important role in antitumor and antiangiogenic activity through the increase in the production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-27.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2565723 (BioLegend Cat. No. 676902)
Structure: A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40 related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution: Monocytes, macrophages, and dendritic cells (DCs).
Function: T cell proliferation and differentiation.
Interaction: Activated T cells, resting natural killer cells (NK), resting NKT cells, neutrophils, eosinophils, mast cells, hepatic stellate cells, hepatoma cells, and hepatocytes.
Ligand/Receptor: IL-27RA, also known as WSX-1 or TCCR, paired with gp130.
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Pflanz S, et al. 2002. Immunity 16:779. 2. Owaki T, et al. 2005. J. Immunol. 175:2191. 3. Batten M, et al. 2006. Nat. Immunol. 7:929. 4. Villarino AV, et al. 2006. J. Immunol. 176:237. 5. Stumhofer JS, et al. 2007. Nat. Immunol. 8:1363. 6. Awasthi A, et al. 2007. Nat. Immunol. 8:1380. 7. Pot C, et al. 2009. J. Immunol. 183:797. 8. Bender H, et al. 2009. Hepatology 50:585. 9. Bosmann M and Ward PA. 2013. J. Leukoc. Biol. 94:1159.
Gene ID: 246778
UniProt: View information about IL-27 on UniProt.org
Clone: B0315A10
Regulatory Status: RUO
Other Names: Interleukin-27 Subunit Alpha, IL-27 (28K subunit)
Isotype: Mouse IgG2b, κ