Biolegend, 677202, Purified anti-MERTK (Mer) Antibody, 100μg

MERTK is a receptor tyrosine kinase of the TAM (Tyro3, Axl, MERTK) family, which preferentially is expressed by macrophages, dendritic cells, and natural killer cells. It plays an important role in processes such as macrophage activation, apoptotic cell engulfment, platelet aggregation, and clot stability. MERTK can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif. The downstream signaling components include PI3 kinase, PLCγ, and MAP kinase. MERTK is upregulated in a wide variety of cancers including leukemia, non-small cell lung cancer, glioblastoma, melanoma, prostate cancer, breast cancer, colon cancer, and gastric cancer.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of recombinant partial-length human MERTK.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Graham DK, et al. 2006. Clin. Cancer Res. 9:2662. Sather S, et al. 2007. Blood 109:1026.
RRID: AB_2565900 (BioLegend Cat. No. 677202)
Structure: 999 amino acids with a predicted molecular weight of approximately 110 kD with the characteristic of the common NCAM (neural adhesion molecule)-related extracellular domain. Observed molecular weight is 140-160 kD due to different glycosylation patterns.
Distribution: Transmembrane protein. Preferentially expressed by macrophages, dendritic cells, natural killer cells, osteoclasts, Sertoli cells, and retinal pigmental epithelium cells.
Function: Macrophage activation, apoptotic cell engulfment, platelet aggregation, and clot stability.
Interaction: TNK2, LGALS3, TUB, TULP1, GAS6, and VAV1.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References: 1. Pierce AM, et al. 2014. Brain Res. 1542:206. 2. Cummings CT, et al. 2013. Clin. Cancer Res. 19:5275. 3. Nguyen KQ, et al. 2013. Autoimmunity 46:294. 4. Qingxian L, et al. 2010. Front Biol. 5:227. 5. Linger RM, et al. 2008. Adv. Cancer Res. 100:35. 6. Lemke G, et al. 2008. Nat. Rev. Immunol. 8:327. 7. Graham DK, et al. 2006. Clin. Cancer Res. 9:2662. 8. Sather S, et al. 2007. Blood 109:1026.
Gene ID: 10461
UniProt: View information about MERTK on UniProt.org
Clone: A311F9G3E1
Regulatory Status: RUO
Other Names: MER Receptor Tyrosine Kinase, c-Mer Proto-Oncogene Tyrosine Kinase, Proto-Oncogene C-Mer, Receptor Tyrosine Kinase MerTK, STK Kinase, MER, RP38, c-Eyk, c-mer, Tyro12, MER-TK
Isotype: Mouse IgG1, κ