Biolegend, 677502, Purified anti-PCSK9 Antibody, 100μg

Proprotein convertase subtilisin/kexin 9 (PCSK9) possesses a signal peptide, a prosegment, a catalytic domain, a hinge region, and a C-terminal Cys-His-rich domain. This protein is primarily synthesized and secreted by hepatocytes. Besides the liver, it is also expressed in the small intestine, kidney, and brain. It is also present in the plasma. Upon translocation to the endoplasmic reticulum, the prosegment of PCSK9 is autocatalytically cleaved and secreted as a stable, enzymatically inactive, non-covalent complex. Elevated low density lipoprotein-cholesterol (LDLc) level is a major risk factor for cardiovascular disease and atherosclerosis. PCSK9 is a regulator of LDLc levels through the binding of LDLR, which subsequently leads to the degradation of LDLR. PCSK9 null mice fed with a high fat diet demonstrated a four-fold decrease in cholesterol accumulation, which stresses the important role played by PCSK9 in the development of cardiovascular disesases. PCS59 also binds to other LDLR family members such as very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER), and apolipoprotein receptor 2 (LRP8/APOER2), which leads to their degradation in the intracellular acidic compartments. Like the LDLR, gene expression of PCSK9 is positively regulated by sterol regulatory element-binding protein 2 (SREBP-2), which is a transcription factor that is activated in response to cellular cholesterol depletion.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse PCSK9, amino acids Gln35-Gln694, expressed with a C-terminal His tag and a linker sequence in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Sun H, et al. 2018. J Lipid Res. 59:207. PubMed
RRID: AB_2565908 (BioLegend Cat. No. 677502)
Structure: Monomer. The molecular mass of a full-length and mature protein is 74.8 kD and 53.7 kD, respectively.
Distribution: Hepatocytes, renal cells, neuroepithelioma, and Schwann cells.
Function: PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and therefore, LDLc levels. PCSK9 is regulated by SREBP-2.
Interaction: Hepatocytes.
Ligand/Receptor: LDLR, VLDLR, APOB, LRP1, and LRP8.
Biology Area: Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: Enzymes and Regulators
Antigen References: 1. Seidah NG, et al. 2003. Proc. Natl. Acad. Sci. USA 100:928.2. Naureckiene S, et al. 2003. Arch. Biochem. Biophys. 420:55.3. Costet P, et al. 2008. Trends Biochem. Sci. 33:426.4. Grefhorst A, et. al. 2008. J. Lipid Res. 49:1303.5. Tavori H, et al. 2013. Circulation 127:2403.6. Denis M, et al. 2012. Circulation 125:894.7. Tibolla G, et al. 2011. Nutr. Metab. Cardiovasc. Dis. 21:835.
Gene ID: 100102
UniProt: View information about PCSK9 on UniProt.org
Clone: M1110F3
Regulatory Status: RUO
Other Names: Neural Apoptosis Regulated Convertase 1 (NARC1), proprotein convertase PC9, subtilisin/kexin-like protease PC9, FH3, HCHOLA3, AI415265, AI747682
Isotype: Rat IgG2b, κ