Biolegend, 677602, Purified anti-IL-11 Antibody, 100μg

IL-11, a member of the IL-6 family of cytokines, exerts a wide range of biological effects on various cell types including hematopoietic cells, hepatocytes, adipocytes, neurons, and osteoblasts. IL-11 works synergistically with other growth factors including SCF, IL-4, IL-3, IL-7, IL-12, IL-13, and GM-CSF to stimulate the proliferation of cells from several hematopoietic lineages. Binding of IL-11 to IL-11 receptor (IL-11R) induces membrane bound gp130 homodimerization and triggers the STAT3 phosphorylation by JAK. IL-11 shares the common receptor subunit gp130 with IL-6, IL-27, LIF, OSM, CNTF, CT-1, CLC, and NP. Female mice deficient in IL-11R revealed that IL-11 has an important role in embryonic implantation. In addition, IL-11 shows anti-inflammatory activity in models of inflammatory bowel disease, chemotherapy induced oral mucositis, and inflammatory arthritis. IL-11 and IL-13 are highly expressed in asthmatic airways (Th2 response). IL-11 can inhibit Th1 responses and the production of Th1 cytokines such as IL-12, shifting inflammation in Th2 direction. Elevated IL-11 expression is associated with tumor grade and invasion in gastric cancer. Recombinant human IL-11 has been clinically approved to improve platelet recovery after chemotherapy-induced thrombocytopenia.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-11, 180 amino acids Val20-Leu199 (Accession# P20809.1) was expressed in 293E.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2565909 (BioLegend Cat. No. 677602)
Structure: "Four-helix bundle" protein that folds similarly to IL-6. Canonical full length human IL-11 contains 199 amino acids with a predicted molecular weight of 21.4 kD. Mouse IL-11 also contains 199 amino acids and has a predicted molecular weight of 21.5 kD.
Distribution: IL-11 can be secreted by various cell types such as epithelial, endothelial, keratinocytes, stromal, neuronal, fibroblasts, osteoclasts, and bone marrow stromal cells.
Function: IL-11, a multifunctional cytokine, plays an important role in processes such as hemopoiesis, thrombopoiesis, megakaryocytopoiesis, and bone resorption. It regulates macrophage differentiation and confers mucosal protection after chemotherapy and radiation therapy. IL-11 expression can also be up-regulated by oncogenic Ras or respiratory virus infections. IL-11 and IL-11Rα are induced by IL-13.
Interaction: Hematopoietic cells, hepatocytes, adipocytes, neurons, osteoblasts, fibroblasts, and gastrointestinal epithelial cells.
Ligand/Receptor: Membrane bound or soluble IL-11Rα heterodimer with gp130β.
Cell Type: B cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Putoczki and Ernst M. 2010. J. Leuko. Biol. 88:1109.2. Wilde MI and Faulds D. 1998. BioDrugs 10:159.3. Barton VA. 2000. J. Biol. Chem. 275:36197.4. Robb L, et al. 1998. Nat. Med. 4:303.5. Lemoli RM, et al. 1995. Br. J. Haematol. 91:319.6. Elias JA, et al. 1994. J. Biol. Chem. 269:22261.7. Chen Q, et al. 2005. J. Immunol. 174:2305.
Gene ID: 3589
UniProt: View information about IL-11 on UniProt.org
Clone: M3103F11
Regulatory Status: RUO
Other Names: Adipogenesis Inhibitory Factor (AGIF)
Isotype: Mouse IgG2a, κ