Biolegend, 677701, Purified anti-mCherry Antibody, 25μg

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins 1,2 . mCherry derives from proteins initially isolated from Cnidarians .
25μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: mCherry.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg per ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Ra EA, et al. 2023. STAR Protoc. 102609:4. PubMed Yoo TY, et al. 2021. J Cell Biol. 220:. PubMed Fote GM, et al. 2022. J Cell Sci. 135:. PubMed Cottier S, et al. 2021. J Cell Sci. :. PubMed
RRID: AB_2801131 (BioLegend Cat. No. 677701) AB_2565910 (BioLegend Cat. No. 677702)
Structure: 236 amino acids with a predicted molecular weight of approximately 27 kD.
Function: Fluorescent protein.
Biology Area: Cell Biology
Antigen References: 1. Shaner NC, et al. 2008. Nat. Methods 5:545. 2. Shaner NC, et al. 2005. Nat. Methods 2:905.
Gene ID: NA
UniProt: View information about mCherry on UniProt.org
Clone: 8C5.5
Regulatory Status: RUO
Other Names: DSRED
Isotype: Mouse IgG2a, κ