Biolegend, 678002, Purified anti-STAT3 Antibody, 100μg

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC, ICC, IP, KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Jafari S, et al. 2023. Bioimpacts. 13:51. PubMed Peng HY, et al. 2023. Signal Transduct Target Ther. 415:8. PubMed Voshagh Q, et al. 2024. Immun Inflamm Dis. e1224:12. PubMed Das J, et al. 2024. Front Immunol. 1325161:15. PubMed Jafari S, et al. 2020. Daru. 28:159. PubMed Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed
RRID: AB_2861053 (BioLegend Cat. No. 678001) AB_2565913 (BioLegend Cat. No. 678002)
Structure: 770 amino acids with a predicted molecular weight of 88.1 kD that contains a C-terminal SH2 domain responsible for homo- or herterodimerization.
Distribution: Cytoplasm.
Function: Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
Interaction: STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lufei C, et al. 2003. EMBO J. 22:1325. 2. Deo D, et al. 2002. J. Biol. Chem. 277:21237. 3. Pfeffer L, et al. 1997. Science 276:1418. 4. Akira S, et al. 1994. Cell 77:63.
Gene ID: 6774
UniProt: View information about STAT3 on UniProt.org
Clone: 4G4B45
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype: Mouse IgG1, κ