Biolegend, 678451, Purified anti-HSPH1 Antibody, 25μg

The HSPH1 protein, also known as heat shock protein 105 (HSP105), is a member of the heat shock protein (HSP) family, which plays a crucial role in the maintenance of cellular homeostasis, especially under stress conditions. HSPH1 is a prominent member of the HSP110 family, which is closely related to the HSP70 family but has unique properties and functions. HSPH1 helps to mitigate these harmful effects by functioning as a molecular chaperone, ensuring that denatured proteins refold correctly or are directed towards degradation pathways. This protective mechanism is vital for preventing the accumulation of protein aggregates, which can be toxic and lead to cell death. Studies have shown that HSPH1 can interact with other heat shock proteins, such as HSP70 and HSP40, to form a functional complex that enhances its chaperone activity. Through these interactions, HSPH1 not only prevents protein misfolding but also facilitates the disaggregation of protein aggregates, promoting cellular recovery from stress. HSPH1 is also implicated in various diseases, particularly those associated with protein misfolding and aggregation. For example, neurodegenerative disorders like Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease are characterized by the accumulation of misfolded protein aggregates in neurons. The upregulation of HSPH1 has been suggested as a protective mechanism in these conditions, as it may help reduce the burden of toxic aggregates. HSPH1 has been found to be overexpressed in various cancers, including breast, lung, and colon cancers, where it contributes to the stabilization of oncogenic proteins and supports the survival of cancer cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human HSPH1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). It is not recommended to use this product in immunocytochemistry (ICC).
RRID: AB_3675194 (BioLegend Cat. No. 678451) AB_3675194 (BioLegend Cat. No. 678452)
Structure: HSPH1 is an 858 amino acid length protein with a predicted molecular weight of 96.9 kD. The expected molecular weight in SDS-PAGE gel is at 110 kD.
Distribution: Expressed in cytosol in most tissues
Function: Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release
Interaction: Interacts with HSPA8/HSC70, HSPA1A, and HSPA1B
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family: Heat Shock Proteins
Antigen References: Easton D P, et al. 2000. Cell Stress Chaperones. 5(4):276-90. Schuermann J P, et al. 2008. Mol. Cell. 31(2):232-43. Canpanella C, et al. 2018. Int. J. Mol. Sci. 19(9):2603
Gene ID: 10808
UniProt: View information about HSPH1 on UniProt.org
Clone: W20247H
Regulatory Status: RUO
Other Names: Heat Shock Protein Family H Member 1, HSP110, HSP105, Heat Shock 105kDa/110kDa Protein 1, HSP105A, HSP105B, Antigen NY-CO-25, NY-CO-25
Isotype: Rat IgG2b, κ