Biolegend, 680102, Purified anti-HDAC2 Antibody, 100μg

HDAC2 is a member of the class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups from the side chain of lysine residues on the N-terminal region of core histones. HDAC2 lacks DNA binding activity and executes its function by recruiting transcription factors and forming large transcriptional repressor complexes. In addition to histones, HDAC2 can deacetylate transcription factors and modify their transcriptional activity. HDAC2 is highly homologous to HDAC1 in regulating cell proliferation, differentiation, and apoptosis. HDAC2 has been shown to negatively regulate synaptic plasticity and memory formation. Elevated activity of HDAC2 has been associated with the loss of memory and neuronal degeneration.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human HDAC2 peptide (471-488 a.a.) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP, ICC, KO/KD-WB, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10 - 30 μg per ml. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: A minor non-specific band of ~70 kDa was observed by western blot during product development.
RRID: AB_2566332 (BioLegend Cat. No. 680101) AB_2566332 (BioLegend Cat. No. 680102)
Structure: 488 amino acids with a predicted molecular weight of 55.4 kD. It contains a histone deacetylase domain.
Distribution: Nucleus.
Function: HDAC2 is a histone deacetylase responsible for the deacetylation of ε-amino acid group of lysine residues on the N-terminal tail of core histones. Forms of complex with transcription factors and acts as a transcriptional co-repressor.
Interaction: Forms histone deacetylase complexes with HDAC1, RBBP4, and RBBP7. Interacts with GFI1, SNW1, HDAC7, PRDM6, SAP30, SETDB1, SUV39H1, H2AFY, ATR, CBFA2T3, BCL-6, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A, and PELP1.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References: 1. Winter M, et al. 2013. EMBO J. 32:3176. 2. Ma P, et al. 2013. PLoS Genet. 9:e1003377. 3. Kurita M, et al. 2012. Nat. Neurosci. 15:1245. 4. Segre CV, et al. 2011. J. Biomed. Biotechnol. 2011:690848. 5. Brunmeir R, et al. 2009. Int. J. Dev. Biol. 53:275. 6. Guan JS, et al. 2009. Nature 459:55.
Gene ID: 3066
UniProt: View information about HDAC2 on UniProt.org
Clone: 13G8C67
Regulatory Status: RUO
Other Names: Histone Deacetylase 2, YY1-Associated Factor 1, HD2, Transcriptional Regulator Homolog RPD3, YAF1
Isotype: Mouse IgG1, κ