Biolegend, 680402, Purified anti-IGF1 Antibody, 100μg

IGF-I, also known as insulin-like growth factor 1), was initially described in 1957 as a hormone that is important for child development, and also has anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50% similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I; pro-IGF-I is processed to the mature, active protein, and this procedure requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven IGF binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half-lives and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs in serum. 75% of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated with growth hormone insensitivity syndrome (GHIS), which is also called Laron syndrome, liver cirrhosis, age-related cardiovascular, neurological diseases, and intrauterine growth restriction.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human IGF-I, amino acids (Gly49-Ala118) (Accession# PO05019), expressed in E. coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2566356 (BioLegend Cat. No. 680402)
Structure: The 70 amino acid recombinant protein has a predicted molecular weight of approximately 7.6 kD. The predicted N terminal amino acid is Gly.
Distribution: Secreted protein.
Function: Similar to insulin in function and structure and is involved in mediating growth and development.
Interaction: IGF binding protein 1, IGF binding protein 2, IGF binding protein 3, IGF binding protein 4, IGF binding protein 5, IGF binding protein 6, and Fibronectin 1.
Ligand/Receptor: IGF1R.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Bell GI, et al. 1984. Nature 310:775.2. Karey KP, Sirbasku DA. 1988. Cancer Res. 48:4083.3. Mohan S, Baylink DJ. 2002. J. Endocrinol. 1:19.4. Ostrovsky O, et al. 2010. Biochim. Biophys. Acta. 1803:333.5. Barton ER, et al. 2012. FASEB. J. 26:3691.6. Puche JE, Castilla-Cortazar I. 2012. J. Transl. Med. 10:224.7. Ni F, et al. 2013. Nat. Commun. 4:1479.
Gene ID: 3479
UniProt: View information about IGF1 on UniProt.org
Clone: 1500102
Regulatory Status: RUO
Other Names: Insulin-Like Growth Factor 1, IGF-1, Mechano Growth Factor, Somatomedin-C, IGF-I, MGF, Insulin-Like Growth Factor IA, Insulin-Like Growth Factor IB, Nonsuppressible insulin-like activity I (NSILA-I)
Isotype: Mouse IgG1, κ