Biolegend, 680602, Purified anti-V5-tag Antibody, 100μg

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.
100μg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Bussey KA, et al. 2018. PLoS Pathog. 11:43. PubMed Hertzog J, et al. 2022. EMBO J. :e109217. PubMed Pham TH, et al. 2020. Cancer Discov. . PubMed Lou Y, et al. 2020. Cell Mol Immunol. 1.439583333. PubMed Stempel M, et al. 2019. EMBO J. 38:. PubMed
RRID: AB_2566386 (BioLegend Cat. No. 680601) AB_2566387 (BioLegend Cat. No. 680602)
Structure: 14 amino acid peptide (GKPIPNPLLGLDST)
Function: The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
Biology Area: Cell Biology
Antigen References: 1. Uhlen M, et al. 1990. Meth Enzymol. 185:129. 2. Hanke T, et al. 1995. J. Virol. Methods 53:149. 3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551. 4. Thomas SM, et al. 1988. Cell 54:891.
Gene ID: NA
UniProt: View information about V5-tag on UniProt.org
Clone: 7/4
Regulatory Status: RUO
Other Names: GKPIPNPLLGLDST epitope tag
Isotype: Mouse IgG2b, κ