Biolegend, 681451, Purified anti-CD142 Antibody, 25μg

CD142, also known as Tissue Factor (TF), Coagulation Factor III, and Thromboplastin, is a 45 kD type I transmembrane glycoprotein. It is expressed on the surface of a variety of cells that are physically separated from the circulating blood which include smooth muscle cells, fibroblasts, keratinocytes, glomerular epithelial cells (cytoplasmic inclusions), astrocytes, myocardium, liver stromal cells, pancreas cells, and is also expressed on activated monocytes and stimulated endothelial cells. CD142 is a high-affinity receptor for coagulation factor VII/VIIa and initiates the extrinsic pathway of blood coagulation. CD142 also plays an important role in a variety of diseases such as sepsis, atherosclerosis, and cancer.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human CD142
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone A22071J) is not recommended for immunocytochemistry (ICC) or Immunohistochemistry in formalin-fixed paraffin-embedded tissue (IHC-P).
RRID: AB_3662336 (BioLegend Cat. No. 681451) AB_3662336 (BioLegend Cat. No. 681452)
Structure: 45 kD Type I transmembrane glycoprotein
Distribution: Smooth muscle cells, fibroblasts, keratinocytes, glomerular epithelial cells, astrocytes, myocardium, liver stromal cells, pancreas cell, stimulated endothelial cells, stimulated monocytes
Function: Blood coagulation
Ligand/Receptor: Coagulation Factor VIIa
Cell Type: Astrocytes, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Carson SD, et al. 1994. Blood. 84:526-34. McComb RD, et al. 1991. Am J Pathol. 139:491-4. Whittle SM, et al. 1995. Thromb Res. 79:451-9.
Gene ID: 2152
UniProt: View information about CD142 on UniProt.org
Clone: A22071J
Regulatory Status: RUO
Other Names: Coagulation Factor III, Tissue Factor, TF, Thromboplastin, platelet tissue factor, factor III, factor 3, F3, F-3, TFA, P13726
Isotype: Mouse IgG2b, κ