Biolegend, 682102, Purified anti-HSC70 (HSPA8) Antibody, 100μg

Heat shock cognate 71 kD protein or HSC70 (HSPA8) is one of the major constitutively expressed, cognate protein chaperones to supply a multitude of the housekeeping chaperoning functions. As a chaperone, HSC70 binds to nascent polypeptides to facilitate correct folding. Besides the chaperone functions, secreted HSC70 is involved in inflammatory signal pathways via extracellular interaction with TLR2/TLR4. It also plays a central role in modulating antigen transport within cells to control MHC class II presentation during nutrient stress. Furthermore, HSC70 may serve as a molecular switch to modulate endocytic and autophagy pathways.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant human HSC70 fragment (1-1755bp of Acc. Y00371)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2566574 (BioLegend Cat. No. 682102)
Structure: 646 amino acids with a predicted molecular weight of approximately 71 kD.
Distribution: Cytoplasm, nucleus, and cell membrane.
Function: Chaperone and antigen transport.
Interaction: PACRG, HSPH1/HSP105, IRAK1BP1, BAG1, DNAJC7, DNAJB14, E3 ligase CHIP, CITED1 , CDC5L, PLRG1 and BCAS2, SV40 VP1, TRIM5, METTL21A, PARK2, and FOXP3.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors
Molecular Family: Heat Shock Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Deffit SN, Blum JS. 2015. Mol. Immunol. 68:85-8. 2. Pratt WB, et al. 2015. Annu. Rev. Pharmacol. Toxicol. 55:353. 3. Stricher F, et al. 2013. Autophagy 9:1937. 4. Liao Y, et al. 2014. Curr. Pharm. Des. 20:101. 5. Höhfeld J. 1998. Biol. Chem. 379:269.
Gene ID: 3312
UniProt: View information about HSC70 on UniProt.org
Clone: 9/2
Regulatory Status: RUO
Other Names: Heat Shock 70 kD Protein 8, HSP70, HSP73, HSPA10, Heat Shock Cognate Protein 54, HSC54, heat stress cognate 70
Isotype: Mouse IgG2b, κ