Biolegend, 683102, Purified anti-RORα Antibody, 100μg

Retinoid related orphan receptor alpha, also known as RORα, is a NR1 Thyroid Hormone Like Receptor. Three different subtypes have been discovered: RORα, RORβ, and RORγ. RORα plays a key role in the development of the cerebellum. The deletion of RORα in mice causes severe impairment in the differentiation of cerebellar Purkinje neurons and results in the "staggerer" phenotype. RORα has been suggested to participate in the regulation of a number of other physiological processes, which include bone formation. Recently, RORα was reported to regulate lymphocyte development and mediate Th17 differentiation synergistically with RORγ.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant protein (isoform 1, 101-308 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: RORa has two isoforms, 53 kD and 59 kD, and this antibody recognizes both isoforms. This antibody does not recognize human.
Product Citations: Shearn CT, et al. 2023. PLoS One. e0290385:18. PubMed
RRID: AB_2566748 (BioLegend Cat. No. 683102)
Structure: 523 amino acids with a predicted molecular weight of 59 kD. RORs contain four major functional domains: an amino-terminal domain, DNA domain (DBD), a hinge domain, and a ligand-binding domain (LBD).
Distribution: Nucleus.
Function: RORα can bind as a monomer or homodimer to hormone response elements upstream of several genes to enhance the expression of those genes.
Interaction: RORα interacts with p300, beta-catenin, HIF1A, NCOA2, CEBPB, FOXP3, and NRIP1.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Giguere V, et al. 1994. Genes Dev. 8:538. 2. Atkins GB, et al. 1999. Mol. Endocrinol. 13:1550. 3. Dzhagalov I, et al. 2004. 173:2952. 4. Yang XO, et al. 2008. Immunity 28:29. 5. Lee JM, et al. 2012. Mol. Cell 48:572. 6. Solt LA, et al. 2012. Trends Endocrinol Metab. 23:619.
Gene ID: 6095
UniProt: View information about RORalpha on UniProt.org
Clone: 6G4C18
Regulatory Status: RUO
Other Names: Retinoid related orphan receptor alpha, RORA, Nuclear receptor RZR-alpha, Nuclear receptor subfamily 1 group F member 1 (NR1F1), Nuclear receptor ROR-alpha
Isotype: Mouse IgG2b, κ