Biolegend, 683402, Purified anti-PPARγ Antibody, 100μg

PPARγ, also known as NR1C3, is a ligand-dependent transcription factor belonging to the peroxisome-proliferator activated receptor (PPAR) family. Upon activation by its ligands, such as fatty acids and prostaglandin PGJ2, PPARγ forms heterodimers with the retinoid X receptors (RXRs) and binds to DNA specific PPAR response elements (PPRE). In the absence of ligand, the PPARγ/RXR heterodimer represses its target genes by recruiting and stabilizing the corepressor complexes at the promoter regions. PPARγ is expressed most abundantly in adipose tissue and induced during adipocyte differentiation. PPARγ has been suggested to regulate the expression of key genes involved in glucose metabolism, lipid storage, energy homeostasis, adipogenesis, atherosclerosis, inflammation, and cancer.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic PPARγ peptide conjugated to KLH (CETDMSLHPLLQEIYKDLY)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
RRID: AB_2572020 (BioLegend Cat. No. 683402)
Structure: 505 amino acids with an approximate molecular weight of 57.6 kD. Contains two zinc finger domains responsible for DNA binding.
Distribution: Nucleus.
Function: PPARγ is a nuclear receptor that modulates transcription of its target genes. It plays crucial roles in inflammation, energy homeostasis, and adipocyte differentiation.
Interaction: PPARγ interacts with FOXO1, RXRA, PPARBP, ASXL1, ASXL2, DNTTIP2, FAM120B, MAP2K1/MEK1, NR0B2, PDPK1, PRDM16, PRMT2 and TGFB1I1.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Majithia AR, et al. 2014. Proc. Natl. Acad. Sci. USA 111:13127. 2. Aprile M, et al. 2014. PPAR. Res. 2014:537865. 3. Pott S, et al. 2013. PLoS One 7:e48102. 4. Klemke M, et al. 2011. Cancer Genet. 204:334. 5. Folwaczny M, et al. 2011. Innate Immun. 17:541. 6. Fuentes P, et al. 2010. J. Endocrinol. 206:75.
Gene ID: 5468
UniProt: View information about PPARgamma on UniProt.org
Clone: 14G4B17
Regulatory Status: RUO
Other Names: Peroxisome proliferator-activated receptor gamma, PPAR gamma, NR1C3
Isotype: Mouse IgG1, κ