Biolegend, 684302, Purified anti-Lck Phospho (Tyr505) Antibody, 100μg

The Src family tyrosine kinase p56 Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complex results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at Tyrosine 505 negatively regulates Lck, and is proposed to generate a closed, inactive conformation of the protein.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Lck peptide phosphorylated at Tyr 505.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml (1:250-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is predicted to recognize both 58 and 61 kDa isoforms based off the presence of the immunizing epitope in both proteins. This clone may also recognize certain phosphorylated SRC tyrosine kinase family members due to high sequence similarity.
RRID: AB_2716046 (BioLegend Cat. No. 684301) AB_2716047 (BioLegend Cat. No. 684302)
Structure: 509 and 539 amino acid isoforms with predicted molecular weights of 58 and 61 kD, respectively. Contains N-terminal domain with myristylation and palmitoylation sites, a kinase domain, and SH2 and SH3 domains that mediate protein-protein interactions bet
Distribution: T cells
Function: T cell receptor signaling
Interaction: AXL, CD2, CD4, CD5, CD8, CD44, CD45, CD122, PI4K, VAV1, RASA1, FYB, CDK1, RAF1, ZAP70, SYK, RUNX3
Cell Type: T cells
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Philipsen L, et al. 2017. Sci Signal. eaaf4736 2. Moogk D, et al. 2016. J. Immunol. 197:644. 3. Klammt C, et al. 2015. Nat. Immunol. 16:961. 4. Cancer Genome Atlas Network. 2015. Cell. 161:1681. 5. Casas J, et al. 2014. Nat. Commun. 5:5624. 6. McNeill L, et al. 2007. Immunnity. 27:425. 7. Lefebvre DC, et al. 2003. Biochem. Biophys. Acta. 1650:40. 8. Kabouridis PS. 2003. Biochem J. 371:907.
Gene ID: 3932
UniProt: View information about Lck Phospho Tyr505 on UniProt.org
Clone: A17013B
Regulatory Status: RUO
Other Names: Leukocyte C-terminal Src kinase, Protein YT16, p56-LCK, T cell-specific protein-tyrosine kinase
Isotype: Mouse IgG2b, κ