Biolegend, 685202, Purified anti-HDAC3 Antibody, 100μg

HDAC3, also known as RPD3-2, HDAC-3, and SMAP45, is a histone deacetylase and is responsible for the deacetylation of lysine residues on core histones and other non-histone substrates. The deacetylation of histones is important for transcriptional regulation, cell cycle progression, and other developmental related functions. HDAC3 represses gene expression by participating in the transcriptional repressor activity of BCL6, by deacetylating H3K27 on enhancer elements. It is also related to participating in transcriptional regulation through binding to YY1, which is a a zinc-finger transcription factor.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human HDAC3 recombinant protein (405-424 a.a.) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2861069 (BioLegend Cat. No. 685201) AB_2572203 (BioLegend Cat. No. 685202)
Structure: 428 amino acids with a predicted molecular weight of 49 kD. It belongs to the class I histone deacetylase family.
Distribution: Nucleus.
Function: HDAC3 is a deacetylase responsible for the deacetylation of lysine resides on core histones and other non-histone proteins.
Interaction: Forms heterodimer with YY1. Interacts with HDAC7, 9, 10, DACH1, DAXX, BCOR, MJD2A/JHDM3A, NRIP1, PRDM6, and SRY.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: 1. Hua G, et al. 2016. Proc Natl Acad Sci USA. 113:E626. 2. Choi HK, et al. 2015. Nat. Commun. 6:8225. 3. Kim WY, et al. 2015. Int. J. Mol. Med. 35(1):202-10. 4. McHugh CA, et al. 2015. Nature 521:232. 5. Sun Z, et al. 2013. Mol. Cell. 52:769. 6. Sun Z, et al. 2012. Nat. Med. 18:934.
Gene ID: 8841
UniProt: View information about HDAC3 on UniProt.org
Clone: W15097A
Regulatory Status: RUO
Other Names: Histone deacetylase 3, HD3, SMAP45, RPD3-2
Isotype: Mouse IgG2b, κ