Biolegend, 686302, Purified anti-FOXO3 Antibody, 100μg

The Fork-head box O (FOXO) family of transcription factors is comprised of four family members; FOXO1, FOXO3, FOXO4, and FOXO6. FOXO3 was originally identified as a tumor suppressor and has been shown to regulate several cellular processes, such as cell cycle arrest, apoptosis, autophagy, longevity, and stress response. FOXO3 activity is determined by its post-translational modifications and subcellular localization. FOXO3 is inactivated by cytoplasmic retension through AKT/PI3K signaling pathways. FOXO3 inactivation has been associated with cell proliferation and tumorigenesis. In contrast, over-expression of FOXO3 leads to inhibition of tumor growth. FOXO3 also plays an important role in the development of regulatory T cells. FOXO3-deficient mice showed organ inflammation phenotype and increased production of proinflammatory cytokines during viral infection.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human FOXO3 recombinant protein (424-673 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2616882 (BioLegend Cat. No. 686302)
Structure: 673 amino acids with a predicted molecular weight of 71.3 kD. Contains a fork-head domain responsible for DNA binding.
Distribution: Cytoplasm and nucleus.
Function: FOXO3 is a transcription factor, participating in the regulation of cell cycle arrest, apoptosis, autophagy, longevity, and stress response.
Interaction: Interacts with SIRT2, 14-3-3 beta, 14-3-3 zeta, STK4, PIM1, and DDIT3.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Morris BJ, et al. 2015. Gerontology 61:515. 2. Larsen SK, et al. 2014. Oncoimmunology 3:e953411. 3. Eijkelenboom A, et al. 2013. Cell Rep. 5:1664. 4. Yeo H, et al. 2013. EMBO J. 32:2589. 5. Pelosi E, et al. 2013. Nat. Commun. 4:1843. 6. Litvak V, et al. 2012. Nature 490:421.
Gene ID: 2309
UniProt: View information about FOXO3 on UniProt.org
Clone: W15111A
Regulatory Status: RUO
Other Names: Forkhead box O3, FKHRL1, FOXO3A
Isotype: Mouse IgG2a, κ