Biolegend, 687452, Purified anti-Aurora A Antibody, 100μg

Aurora A (AURKA) is a serine/threonine kinase and a crucial regulator of mitosis. It is a member of the Aurora kinase family, which plays key roles in cell cycle regulation, spindle formation, and chromosome segregation. AURKA is highly conserved among eukaryotic organisms and is essential for maintaining genomic stability. Aurora A is predominantly active during the G2/M phase of the cell cycle which promotes the recruitment of pericentriolar material (PCM) to centrosomes, facilitating their maturation. Aurora A phosphorylates and activates several substrates involved in mitotic entry, such as PLK1 (Polo-like kinase 1) and CDC25B which enable the activation of cyclin-dependent kinases, driving the cell into mitosis. Aurora A stabilizes spindle microtubules by interacting with proteins such as TPX2 (Targeting Protein for Xklp2) and ensures proper bipolar spindle formation facilitating a prerequisite for accurate chromosome alignment and segregation. Aberrant expression or dysregulation of AURKA is implicated in oncogenesis. Overexpression is frequently observed in various cancers, including breast, ovarian, and colorectal cancers.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Aurora A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401) or Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is not recommended.
RRID: AB_3699094 (BioLegend Cat. No. 687451) AB_3699094 (BioLegend Cat. No. 687452)
Structure: Aurora A is a 403 amino acid length protein with a predicted molecular weight of 45.8 kD and contains a Protein kinase domain.
Distribution: Localizes in the cytoplasm or in the centrosome of mitotic cells
Function: Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression and associates with the centrosome and the spindle microtubules during mitosis
Interaction: Forms complex with NEDD9 and CTTN. Interacts with CPEB1, PPP2CA.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family: Organelle Markers, Protein Kinases/Phosphatase
Antigen References: Nikonova AS, et al. 2013. Cell Mol. Life Sci.70:661-87. Mosquera JM, et al. 2013. Neoplasia. 15:1-10. Zheng F, et al. 2016. Nat Commun.7:10180. Du R, et al. 2021. Mol Cancer. 20:15.
Gene ID: 6790
UniProt: View information about Aurora A on UniProt.org
Clone: W23093D
Regulatory Status: RUO
Other Names: Aurora Kinase A, Aurora 2, AURKA, AIK, AIRK1, ARK1, AURA, Aurora/IPL1-related kinase 1, Serine/Threonine-Protein Kinase Aurora-A
Isotype: Rat IgG2a, κ