Biolegend, 687602, Purified anti-Histone H3.1 Phospho (Ser28) Antibody, 100μg

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails. Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Histone H3.1 S28ph peptide (21-39)(ATKAARK(phS)APATGGVKKPH), Freund's complete adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested WB, ChIP - Verified Direct ELISA, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For ChIP applications, the suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2616945 (BioLegend Cat. No. 687602)
Structure: H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution: Nucleus.
Function: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Therefore, histones limit the accessibility of DNA by providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction: Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family: Phospho-Proteins
Antigen References: 1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Yoshimi T, et al. 2013. Monoclon. Immunodiagn. Immunother. 32:119.
Gene ID: 8350
UniProt: View information about Histone H3.1 on UniProt.org
Clone: 5D10D4
Regulatory Status: RUO
Other Names: Histone-H3
Isotype: Rat IgG2a, κ