Biolegend, 688202, Purified anti-Strep-Tag Antibody, 25μg

The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
25μg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic Peptide
Formulation: PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.
Preparation: The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Wang WA, et al. 2023. J Cell Sci. 136:. PubMed Liu Y, et al. 2021. MBio. 12:e0233521. PubMed Dominguez Andres A, et al. 2020. bioRxiv. . PubMed Xu Z, et al. 2022. Front Immunol. 13:1047922. PubMed Xu Z, et al. 2021. Cell Death Dis. 12:57. PubMed
RRID: AB_2629594 (BioLegend Cat. No. 688202)
Structure: The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.
Function: Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.
Biology Area: Cell Biology
Antigen References: 1. Skerra A, et al. 2000. Methods Enzymol. 326:271. 2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528.
Gene ID: NA
UniProt: View information about Strep-Tag on UniProt.org
Clone: BL26882
Regulatory Status: RUO
Isotype: Mouse IgG1, κ