Biolegend, 688751, Purified anti-LAT1/SLC7A5 Antibody, 25μg

L-Type Amino Acid Transporter 1 (LAT1) is crucial for transporting large neutral amino acids like leucine, isoleucine, and phenylalanine across cell membranes, playing a significant role in cellular metabolism and nutrient supply. LAT1 is highly expressed in the blood-brain barrier, where it facilitates the delivery of essential amino acids to the brain. Its regulation involves various mechanisms, including transcriptional and post-transcriptional controls, which ensure its activity aligns with cellular needs. Dysregulation of LAT1 is associated with several diseases, including cancer, where it supports tumor growth by supplying amino acids necessary for rapid cell proliferation. Additionally, LAT1 is implicated in neurological disorders like Alzheimer's and Parkinson's diseases, where it affects the transport of therapeutic agents across the blood-brain barrier.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Fragment of recombinant Human LAT1/SLC7A5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, ICFC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunohistochemistry, a concentration range of 1.25 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using either PFA-Methanol or Methanol only. We do not recommend PFA-Triton X-100 for fixation/permeabilization.For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).For use of this antibody in intracellular Flow Cytometry (ICFC), we recommend fixation/permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3683498 (BioLegend Cat. No. 688751) AB_3683498 (BioLegend Cat. No. 688752)
Structure: LAT1 is a transmembrane protein that consists of 507 amino acids and has a molecular weight of approximately 55 kDa
Distribution: LAT1 is primarily localized in the plasma membrane of cells. It is highly expressed in the blood-brain barrier and the placenta, facilitating the transport of essential amino acids to the brain and fetus. LAT1 is also found in cancer cells, where it supports rapid cell growth and metabolism
Function: • Amino Acid Transport: LAT1 primarily facilitates the transport of large neutral amino acids, such as leucine, isoleucine, and phenylalanine, across cell membranes. This is crucial for cellular metabolism and protein synthesis. • Blood-Brain Barrier: LAT1 is highly expressed in the blood-brain barrier, where it ensures the delivery of essential amino acids to the brain. This is vital for maintaining normal brain function. • Cancer Metabolism: In cancer cells, LAT1 supports rapid cell proliferation by supplying the necessary amino acids for growth and metabolism.
Interaction: • CD98 (SLC3A2), forms a functional heterodimeric complex. This interaction is crucial for LAT1's proper localization and function on the cell membrane, facilitating the transport of large neutral amino acids. • CLDN10, which can influence its function and contribute to disease progression, like in clear cell renal cell carcinoma (ccRCC)
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References: Zhang J, et al. 2020. Front Chem. 8: fchem.2020.564809. Ahmed HS, et al. 2024. Mol Neurobiol. s12035-024-04506-9. Zhao X, et al. 2024. Receptors. 3:474. Scalise M, et al. 2018. Front Chem. 6: fchem.2018.00243.
Regulation: • Transcriptional Control: The expression of LAT1 is regulated at the transcriptional level by various factors, including nutrient availability and cellular stress. • Post-Transcriptional Modifications: LAT1 activity can be modulated through post-transcriptional modifications, such as phosphorylation, which can affect its stability and function. • Interaction with CD98: LAT1 forms a heterodimer with CD98 (SLC3A2), which is essential for its proper localization and function on the cell membrane
Gene ID: 8140
UniProt: View information about LAT1/SLC7A5 on UniProt.org
Clone: W22208B
Regulatory Status: RUO
Other Names: SLC7A5, 4F2lc, CD98Ic, MPE16, Solute Carrier Family 7 Member 5, L-Type Amino Acid Transporter 1
Isotype: Rat IgG2b, κ