Biolegend, 689751, Purified anti-p53 Phospho (Ser15) Antibody, 50microl

The p53 protein is a tumor suppressor transcription factor involved in maintaining genomic stability by regulating the cell cycle, DNA repair, apoptosis, and senescence. It is activated in response to various cellular stresses including DNA damage. Mutations in TP53 gene are found in approximately half of all human cancers, making it one of the most mutated genes in cancer. These mutations often lead to loss of tumor-suppressing functions, contributing to uncontrolled cell proliferation and tumor development. Phosphorylation at Serine 15 is particularly important for p53 activation and function. This modification, often triggered by DNA damage, enhances p53 stability and its ability to activate target genes involved in cell cycle arrest and apoptosis. Without this phosphorylation, p53 ability to respond to stress signals and prevent tumorigenesis is significantly impaired.
50microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide of human p53 phosphorylated at Ser15
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:500 to 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested by western blot using lysates prepared from A431 cells treated with or without ultraviolet light.
RRID: AB_3699095 (BioLegend Cat. No. 689751) AB_3699095 (BioLegend Cat. No. 689752)
Structure: The p53 protein consists of 393 amino acids and has a molecular weight of approximately 53 kDa.
Distribution: Under normal conditions, p53 is present at low levels and shuttles between the nucleus and the cytoplasm. Upon activation by stress signals such as DNA damage, p53 accumulates in the nucleus to initiate DNA repair, cell cycle arrest, or apoptosis. p53 can localize to the mitochondria to promote apoptosis by interacting with pro- and anti-apoptotic protein
Function: • Cell Cycle Arrest: p53 can halt the cell cycle to allow for DNA repair before the cell proceeds to divide • DNA Repair: It activates genes involved in DNA repair mechanisms, ensuring that damaged DNA is corrected • Apoptosis: If the DNA damage is irreparable, p53 can initiate programmed cell death (apoptosis) to prevent the propagation of damaged cells • Senescence: p53 can induce a state of permanent cell cycle arrest known as senescence, preventing damaged cells from dividing • Metabolic Regulation: It also plays a role in regulating cellular metabolism and oxidative stress responses
Interaction: MDM2: This protein is a major negative regulator of p53. It binds to p53, leading to its ubiquitination and degradation
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: Loughery J, et al. 2014. Nucleic Acids Research. 42:7666-80. Marei HE, et al. 2021. Cancer Cell International. 21:703. Ahmadi SE, et al. 2024. Biomarker Research. 12:137. Timofeev O, et al. 2021. Cancers. 13:2422.
Regulation: • Post-Translational Modifications: Phosphorylation, acetylation, and ubiquitination are key modifications that regulate p53 stability and activity • MDM2 Interaction: The protein MDM2 is a major negative regulator of p53. It binds to p53, leading to its ubiquitination and subsequent degradation • Stress Signals: In response to cellular stress such as DNA damage, hypoxia, or oncogene activation, p53 is stabilized and activated to carry out its tumor suppressor functions • Mutations in the TP53 gene, is a common feature in many cancers, leading to uncontrolled cell proliferation and tumor development
Gene ID: 7157
UniProt: View information about p53 on UniProt.org
Clone: Poly24A01
Regulatory Status: RUO
Other Names: P53, Tumor protein 53, LFS1, Tumor suppressor p53, Cellular tumor antigen P53, Antigen NY-CO-13, Phosphoprotein p53
Isotype: Rabbit Polyclonal IgG