Biolegend, 689802, Purified anti-TRAF1 Antibody, 100μg

TNF Receptor Associated Factor 1 (TRAF1) is a member of the TRAF protein family, which mediates the downstream signaling of the TNFR superfamily, such as CD40, CD30, TNFR1 and TNFR2. Upon stimulation of TNF-α, TRAF1 forms a heterodimer with TRAF2 and subsequently activates MAPK/JNK and NFkB pathways. The TRAF1/TRAF2 complex also interacts with apoptosis inhibitor protein IAPs and mediates the anti-apoptotic signaling from TNF receptor. This heterodimer is part of the antiapoptotic E3 ubiquitin-protein ligase complex which promotes ubiquitination of target proteins, such as MAP3K14. TRAF proteins function as adaptor proteins for many cell surface receptors and participate in cell survival, proliferation and differentiation. Defects in TRAF1 have been shown to associate with hodgkin lymphoma and rheumatoid arthritis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human TRAF1 recombinant protein (1-182 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2629790 (BioLegend Cat. No. 689802)
Structure: 416 amino acids with a predicted molecular weight of 46 kD. Contains a C-terminal MATH/TRAF domain responsible for binding to receptor cytoplasmic domains.
Distribution: Cytoplasm.
Function: TRAF1 is an adaptor protein, mediating downstream signaling of the TNFR superfamily. It plays a role in the regulation of cell survival, proliferation, and differentiation.
Interaction: TRAF1 forms a homotrimer and a heterodimer with TRAF2. Interacts with TRAIP, TANK, RIPK2, BIRC2, IRC3, NFATC2IP, TRAFD1, HIVEP3, MAP3K14, and TNFR family members.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Kim CM, et al. 2016. Sci. Rep. 6:25526. 2. Greenfeld H, et al. 2015. PLoS Pathog. 11:e1004890. 3. Lu YY, et al. 2013. Nat. Commun. 4:2852. 4. McPherson AJ, et al. 2012. J. Biol. Chem. 287:23010. 5. Wang C, et al. 2012. J. Exp. Med. 209:77. 6. Lee SY, et al. 2007. Adv. Exp. Med. Biol. 597:25.
Gene ID: 7185
UniProt: View information about TRAF1 on UniProt.org
Clone: W15137A
Regulatory Status: RUO
Other Names: TNF receptor associated factor 1, EBI6
Isotype: Mouse IgG1, κ