Biolegend, 689902, Purified anti-c-Met Antibody, 100μg

The Mesenchymal Epithelial Transition (MET) receptor belongs to a family of Receptor tyrosine kinases (RTKs). The MET proto-oncogene is located on chromosome 7q21–31 and encodes the receptor tyrosine kinase C-Met. It  is a 190 kD glycoprotein heterodimer made up of an extracellular α-chain which is linked by a disulphide bond to a transmembrane β-chain. c-Met is initially synthesised as a 170 kD single polypeptide as precursor that is proteolytically cleaved to form the α-chain and the β-chain. TPR-MET oncogenic fusion protein from human osteosarcoma tumor cells was first discovered in 1984. c-Met is a hepatocyte growth factor (HGF) receptor. The initiation of MET signaling begins with the binding of HGF to the MET receptor at cell membrane that causes C-Met dimerization. Subsequent activation is through a process of trans-phosphorylation of the two tyrosine residues in the catalytical regions Y1234 and Y1235, followed by trans-phosphorylation of two docking tyrosines (Y1349 and Y1356). It enables MET to bind to multiple substrates and activate a variety of signaling pathways, such as MAPK, PI3K-AKT cascades, STAT and NF-κB signaling pathways. This is responsible for driving proliferation, cell survival, migration and invasiveness. c-Met is overexpressed in different human tumors, such as thyroid, gastric, pancreatic, breast, cervical and prostate cancers. Increasing evidence indicated that MET may be a common mechanism of resistance to anticancer treatment such as approved EGFR and VEGFR inhibitors, anti-HER2 therapies and a BRAF inhibitor. Hence, the HGF-MET axis has been targeted  in solid tumors to overcome the drug resistance.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Bacterially expressed human c-Met alpha chain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 12.1 does not cross-react with mouse. Clone 12.1 recognizes the precursor and a chain c-Met; the ß chain will not be recognized by this antibody.
RRID: AB_2629791 (BioLegend Cat. No. 689902)
Structure: Heterodimer made of an alpha chain (50 kD) and a beta chain (145 kD) which are disulfide linked.
Distribution: Membrane; single-pass type I membrane protein.
Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival.
Interaction: Interacts with SPSB1, SPSB2, SPSB3, SPSB4, INPP5D/SHIP1, RANBP9, RANBP10, MUC20, GRB10, PTPN1 and PTPN2. When phosphorylated, interacts with STAT3, PIK3R1, SRC, PCLG1, GRB2, GAB1 and INPPL1/SHIP2.
Ligand/Receptor: HGF.
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Garajová I, et al. 2015. Transl. Oncogenomics 7:13. 2. Cooper CS, et al. 1984. Nature. 311:29. 3. Furge KA, et al. 2000. Oncogene. 19:5582. 4. Ponzetto C, et al. 1994. Cell. 77(2):261-71.
Gene ID: 4233
UniProt: View information about c-Met on UniProt.org
Clone: 12.1
Regulatory Status: RUO
Other Names: MET, Hepatocyte growth factor receptor, HGF receptor
Isotype: Mouse IgG1, κ