Biolegend, 690502, Purified anti-SIRT1 Antibody, 100μg

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuates p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human SIRT1 recombinant protein (548-747 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2629822 (BioLegend Cat. No. 690502)
Structure: 747 amino acids with a predicted molecular weight of 81.6 kD and observed of approximately 120 kD. Contains a sirtuin-type deacetylase domain.
Distribution: Nucleus, cytoplasm, and PML body.
Function: SIRT1 is a NAD-dependent deacetylase involving in the regulation of cellular metabolism and stress responses.
Interaction: Interacts with p53, E1A binding protein p300, HEY2, NF-kB3, Transcription factor HES-1, Forkhead box protein O3A, and PML.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Li L, Bhatia R. 2015. Curr. Opin. Hematol. 22:324. 2. Qiu G, et al. 2015. FEBS Lett. 589:2034. 3. Chen X, et al. 2015. Immunology 145:455. 4. Simmons GE Jr, et al. 2015. Int. J. Mol. Sci. 16:950. 5. Kauppinen A, et al. 2013. Cell Signal. 25:1939. 6. Paraíso AF, et al. 2013. Mol. Neurobiol. 48:681.
Gene ID: 23411
UniProt: View information about SIRT1 on UniProt.org
Clone: W16003A
Regulatory Status: RUO
Other Names: NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1 (SIR2L1), Regulatory protein SIR2 homolog 1, hSIRT1, hSIR2
Isotype: Rat IgG2a, κ