Biolegend, 691204, PE anti-ZAP70 Antibody, 100tests

ZAP70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP70 have been linked to selective T cell defects. The ZAP70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL).
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-peptide surrounding ZAP70 Y319.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Reed JC, et al. 2020. PLoS One. 15:e0236921. PubMed
RRID: AB_2721648 (BioLegend Cat. No. 691203) AB_2721649 (BioLegend Cat. No. 691204)
Structure: Protein tyrosine kinase, contains two SH2 domains. Human Zap70 has three isoforms with predicted MW of 70kD, 56kD, 36kD. Mouse Zap70 has three isoforms with predicted MW of 70kD, 36kD, 35kD.
Distribution: T cells, NK cells.
Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Interaction: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated.
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Chan AC, et al. 1992. Cell. 71:649. 2. Chan AC, et al. 1994. Science. 264:1599. 3. Arpaia E, et al. 1994. Cell. 76:947.
Gene ID: 7535
UniProt: View information about ZAP70 on UniProt.org
Clone: A15114B
Regulatory Status: RUO
Other Names: Zeta chain associated protein kinase, SYK related tyrosine kinase, ZAP-70
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.