Biolegend, 691602, Purified anti-Artemis Antibody, 100μg

Artemis, also known as DCLRE1C, is a nuclear protein belonging to the metallo-β-lactamase superfamily. Artemis has been found to be required for the assembling of B cell antibody genes and T cell receptor genes from individual V, D, and J segments. During V(D)J recombination, Artemis forms a complex with DNA-dependent protein kinase (DNA-‘PK) and cuts the DNA with hairpin structure. Deficiency in Artemis results in blocking of V(D)J recombination and failure to produce mature B and T cells, which is known as the condition of severe combined immune deficiency (SCID). Artemis is also found to be involved in the termini processing step of double-strand break (DSB) repair. Artemis-deficient cells are more sensitive to DSB induced by ionizing radiation or chemical agents such as bleomycin and neocarzinostatin.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Artemis recombinant protein (347-692 aa) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody has a similar affinity to BioLegend clone 13A9C90 for human Artemis by western blot, but does not recognize mouse Artemis
Product Citations: Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed
RRID: AB_2632653 (BioLegend Cat. No. 691602)
Structure: 692 amino acids with a predicted molecular weight of 78.4 kD. Four isoforms produced by alternative splicing.
Distribution: Nucleus.
Function: Artemis is a nuclear protein exhibiting single-strand-specific 5'-3' exonuclease activity and endonuclease activity. It plays crucial roles in V(D)J recombination and DNA repair.
Interaction: Interacts with ATM, BRCA1, PRKDC and TP53BP1.
Biology Area: Cell Biology, DNA Repair/Replication
Antigen References: 1. Chang HH, et al. 2016. Nucleic Acids Res. 44:4991. 2. Volk T, et al. 2015. Hum. Mol. Genet. 24:7361. 3. Waide EH, et al. 2015. J. Immunol. 195:3171. 4. Lee PP, et al. 2013. Clin. Immunol. 149:464. 5. Köcher S, et al. 2012. Nucleic Acids Res. 40:8336. 6. Beucher A, et al. 2009. EMBO J. 28:3413.
Gene ID: 64421
UniProt: View information about Artemis on UniProt.org
Clone: 15E7C90
Regulatory Status: RUO
Other Names: Protein artemis, DNA cross-link repair 1C protein (DCLRE1C), ASCID, SCIDA, SNM1 homolog C (SNM1C)
Isotype: Mouse IgG1, κ