Biolegend, 691802, Purified anti-RPS6 Antibody, 100μg

The eukaryotic ribosome is composed of 40S and 60S subunits, which is involved in the physiological process of protein translation. In many organisms, ribosomal proteins undergo various post-translational modifications, including phosphorylation, acetylation, methylation, O-linked b-N-acetylglucosaminylation, and ubiquitylation. Ribosomal proteinS6 (RPS6), the first identified ribosomal protein which can be phosphorylated, is a component of eukaryotic ribosomal 40S subunit. Functional studies of RPS6 phosphorylation suggested its role in the regulation of protein synthesis, cell proliferation, and glucose homeostasis. The phosphorylation of RPS6 can be triggered by multiple stimuli including growth factors, tumor promoting agents, and mitogens. The inhibition of RPS6 phosphorylation was found mainly under stress conditions such as hypoxia, heat shock, and hyperosmolarity. RPS6 phosphorylation is also commonly used as a marker for neuronal activity and a read out of mammalian target of rapamycin complex 1 (mTORC1) activity.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human S6 peptide.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC, KO/KD-WB, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested.It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Tsai S, et al. 2018. Cell Metab. 28:922. PubMed Napthine S, et al. 2021. Viruses. 13:. PubMed
RRID: AB_2629833 (BioLegend Cat. No. 691802)
Structure: 249 amino acids with a predicted molecular weight of 28.7kD.
Distribution: Cytoplasm and nucleus.
Function: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
Interaction: NCBP1, PASK.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Kressler A, et al. 2010. Biochim. Biophys. Acta. 1803:673. 2. Biever A, et al. 2015. Front. Mol. Neurosci. 8:75. 3. Ventimiglia FA, Wool IG. 1974. Proc. Natl. Acad. Sci. USA 71:350. 4. Lastick SM, et al. 1977. Mol. Gen. Genet. 152:223. 5. Ruvinsky I, et al. 2005. Genes Dev. 19:2199. 6. Meyuhas O. 2008. Int. Rev. Cell. Mol. Biol. 268:1. 7. Meyuhas O. 2015. Int. Rev. Cell. Mol. Biol. 320:41.
Gene ID: NA
UniProt: View information about RPS6 on UniProt.org
Clone: A16009C
Regulatory Status: RUO
Other Names: 40S ribosomal protein S6, Ribosomal Protein S6
Isotype: Mouse IgG2b, κ