Biolegend, 691902, Purified anti-ZAP70 Phospho (Tyr292) Antibody, 100μg

ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Mutation of Y292 to phenylalanine enhanced NFAT induction in response to BCR stimulation, indicating phosphorylation of Y292 may promote association of ZAP-70 with a negative regulatory protein. ZAP-70 has been reported to bind Cbl following TCR stimulation and phosphorylation of Y292 was required for this interaction, as well as for the negative effect of Cbl on signaling.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human ZAP70 peptide phosphorylated at Y292.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Voisinne G, et al. 2022. Nat Immunol. 23:1355. PubMed
RRID: AB_2632759 (BioLegend Cat. No. 691902)
Structure: 619 amino acids with a predicted molecular weight of 70 kD.
Distribution: Cytoplasm and cell membrane.
Function: Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-γ1-dependent and Ras-dependent signaling cascades. Associates with PLC-γ 1.
Interaction: NFAM1, Cbl, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, CD247/CD3Z.
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9166. 2. Arpaia E, et al. 1994. Cell. 76:947. 3. Chan AC, et al. 1994. Science 264:1599. 4. Negishi I, et al. 1995. Nature 376:435. 5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279. 6. Klammt C, et al. 2015. Nat. Immunol. 16:961. 7. Weiss A, et al. 1993. Cell. 73:209. 8. Gong Q, et al. 2001. J. Exp. Med. 194:507. 9. Magnan A, et al. 2001. J. Exp. Med. 194:491.
Gene ID: 7535
UniProt: View information about ZAP-70 Phospho Tyr292 on UniProt.org
Clone: A16038A
Regulatory Status: RUO
Other Names: p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype: Mouse IgG2b, κ