Biolegend, 692051, Purified anti-Calponin 1 Antibody, 25μg

Calponin 1, encoded by the CNN1 gene, is an actin filament-associated regulatory protein found primarily in smooth muscle but also in non-muscle cells. Calponin 1 plays a significant role in regulating the contractility and structural integrity of the cytoskeleton by binding to actin and actin-interacting proteins, modulating their interplay. For example, by binding to actin and inhibiting myosin light chain kinase Calponin 1 reduces the phosphorylation of myosin light chains, preventing excessive contraction and ensuring appropriate smooth muscle response to physiological stimuli. Furthermore, Calponin 1 has been implicated in various physiological functions beyond muscle contraction including vascular tone and wound healing, making it a key player in maintaining homeostasis in various tissues.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Crude human uterus extract
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: - Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 - Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol - 100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization with either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended. This antibody does not cross react with mouse Calponin 1 in Western Blot.
RRID: AB_3683499 (BioLegend Cat. No. 692051) AB_3683499 (BioLegend Cat. No. 692052)
Structure: Calponin 1 is a 297 amino acid protein with a calculated molecular weight of 33.1 kD.
Distribution: Cytoplasmic, cytoskeleton
Interaction: Actin, tropomyosin, myosin, calmodulin
Biology Area: Cardiovascular Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family: Microfilaments
Antigen References: Wu K and Jin J. 2008. Cell Biochem Biophys. 52:139-48. Liu R and Jin JP. 2016. Gene. 585:143-153. Winder SJ and Walsh M. 1993. Cell Signal. 5:677-86.
Gene ID: 1264
UniProt: View information about Calponin 1 on UniProt.org
Clone: CALP
Regulatory Status: RUO
Other Names: Calponin 1, basic, smooth muscle (CNN1), Epididymis Secretory Protein Li 14, SMCC, Sm-Calp, HEL-S-14, Basic calponin, Calponin H1 smooth muscle
Isotype: Mouse IgG1, κ