Biolegend, 692802, Purified anti-RUNX2 Antibody, 100μg

Runt Related Transcription Factor 2 (RUNX2), also known as CBFA1, belongs to the mammalian Runt-related transcription factor (RUNX) family. The RUNX family is comprised of three members: RUNX1, RUNX2, and RUNX3. The RUNX proteins are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. Among these members, RUNX2 contains one Runt domain and specifically expresses in osteoblasts. It plays critical roles in skeletal mineralization, osteoblast differentiation of mesenchymal stem cells, chondrocyte hypertrophy, endothelial cell migration, and vascular invasion of developing bones. Defects in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) and defective endochondral and intramembranous bone formation. In some cases, defects in RUNX2 are exclusively associated with dental anomalies. RUNX2 binds DNA as a monomer or as a subunit of a heterodimeric complex. In osteoblasts, RUNX2 supports transcriptional activation by synergizing with SPEN/MINT. RUNX2 also functions to inhibit KAT6B (MYST4)-dependent transcriptional activation.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human RUNX2 recombinant protein (237-422 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.002 - 0.01 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes multiple isoforms of RUNX2.
Product Citations: Ebata T, et al. 2021. iScience. 24(6):102643. PubMed
RRID: AB_2632769 (BioLegend Cat. No. 692802)
Structure: 521 amino acids with a predicted molecular weight of 56.6 kD. Contains a Runt domain responsible for DNA-binding and protein-protein interaction.
Distribution: Nucleus.
Function: RUNX2 is a transcription factor and has been found to be involved in the regulation of skeletal gene expression, osteoblastic differentiation, and bone morphogenesis.
Interaction: Interacts with Ku70, Ku80, HIVEP3, IFI204, SATB2, HIPK3, DDX5, FOXO1, CCNB1, KAT6A, KAT6B, FOXP3, and TMEM119.
Biology Area: Cell Biology, Transcription Factors
Antigen References: 1. Kidwai F, et al. 2016. Stem Cells. 34:2079. 2. Tandon M, et al. 2016. Oncogene. 35:4730. 3. Owens TW, et al. 2014. Cancer. Res. 74:5277. 4. Sawai CM, et al. 2013. J. Exp. Med. 210:2151. 5. Komori T. 2011. J. Cell. Biochem. 112:750. 6. Westendorff JJ. 2006. J. Cell. Biochem. 98:54-64.
Gene ID: 860
UniProt: View information about RUNX2 on UniProt.org
Clone: W15212A
Regulatory Status: RUO
Other Names: Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1 (CBF-alpha-1), Oncogene AML-3, Osteoblast-specific transcription factor 2 (OSF-2), Polyomavirus enhancer-binding protein 2 alpha A subunit
Isotype: Rat IgG2a, κ