Biolegend, 693153, Alexa Fluor® 488 anti-BAK1 Antibody, 25tests

BAK1 (Bcl-2 homologous antagonist/killer) is a crucial protein involved in regulating apoptosis, or programmed cell death, which is essential for maintaining cellular homeostasis. It functions as a pro-apoptotic factor, promoting cell death in response to various stress signals. BAK1 belongs to the BCL-2 family of proteins and plays a crucial role in the mitochondrial apoptotic process by promoting mitochondrial outer membrane permeabilization, which leads to the release of apoptogenic factors into the cytosol. BAK1 is regulated through interactions with other proteins, such as BCL-2 family members, which can either inhibit or promote its apoptotic activity. Dysregulation of BAK1 has been implicated in several diseases, thus, overexpression of BAK1 can contribute to neurodegenerative and autoimmune diseases by promoting excessive cell death. Conversely, inhibition of BAK1 is associated with the development of cancers, as it allows cells to evade apoptosis and continue proliferating. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 BAK1 Knockout cell line (Horizon Cat. No. HZGHC005962c001)
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human BAK1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3720598 (BioLegend Cat. No. 693153) AB_3720598 (BioLegend Cat. No. 693154)
Structure: BAK1 protein consists of 211 amino acids with a predicted molecular weight of 23 kDa.
Distribution: Mitochondrial outer membrane
Function: • Pro-apoptotic role: BAK1 promotes apoptosis by facilitating mitochondrial outer membrane permeabilization. • Interaction with BCL-2 family: It interacts with other BCL-2 family proteins to regulate apoptosis. • Release of apoptogenic factors: Upon activation, BAK1 helps release factors from the mitochondria that trigger the apoptotic cascade.
Interaction: • BCL-2 and BCL-XL: These anti-apoptotic proteins bind to BAK1, inhibiting its pro-apoptotic activity. • BAX: BAK1 forms heterooligomers with BAX, another pro-apoptotic protein, to promote mitochondrial outer membrane permeabilization. • p53: The tumor suppressor protein p53 interacts with BAK1 in response to cellular stress, enhancing its apoptotic function. • MCL1: This anti-apoptotic protein also interacts with BAK1, regulating its activity. • VDAC2: BAK1 interacts with the mitochondrial voltage-dependent anion channel 2, which is involved in the release of apoptogenic factors
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Mitochondrial Function, Neurodegeneration
Molecular Family: Mitochondrial Markers
Antigen References: Dadsena S, et al. 2021. Biochem Biophys Acta Biomembr. 1863:183716 Shalaby R, et al. 2020. Biomolecules. 10:1638 Ramesh P, et al. 2020. Apoptosis. 25:305 Pena-Blanco A, et al. 2018. FEBS J. 285:416
Regulation: • Protein interactions: BAK1 is regulated through its interactions with pro- and anti-apoptotic BCL-2 family members. • Activation by stress signals: It remains inactive until activated by various cellular stress signals. • Post-translational modifications: Phosphorylation and other modifications can influence BAK1's activity and stability.
Gene ID: 578
UniProt: View information about BAK1 on UniProt.org
Clone: W23097D
Regulatory Status: RUO
Other Names: BCL2 antagonist/killer 1, BCL2L7, BAK, CDN1, Apoptosis regulator BAK, Bcl-2 homologous antagonist/killer
Isotype: Rat IgG2a, κ