Biolegend, 693302, Purified anti-STAT2 Antibody, 100μg

STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT1, p48, and CREB binding protein.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant protein of human STAT2 (671-851 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50 - 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W15088A does not cross-react with mouse (in-house tested).
RRID: AB_2632834 (BioLegend Cat. No. 693302)
Structure: STAT family, contains coiled coil and SH2 domain, 113 kD.
Distribution: Translocates into nucleus after phosphorylation. Expressed in skin, thymus.
Function: Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT.
Interaction: STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein.
Biology Area: Cell Biology, Transcription Factors
Antigen References: 1. Bluyssen HA, Levy DE. 1997. J. Biol. Chem. 272:4600. 2. Qureshi SA, et al. 1996. Mol. Cell. Biol. 16:288.
Gene ID: 6773
UniProt: View information about STAT2 on UniProt.org
Clone: W15088A
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator
Isotype: Mouse IgG2b, κ