Biolegend, 693506, Alexa Fluor® 488 anti-ZAP-70 Antibody, 100tests

ZAP-70 is a 70kD protein expressed near the surface membrane of T-cells and NK cells. It is part of the intracellular signaling mechanism of the T-cell receptor, and plays integral role in T-cell signaling where it interacts with CD3-ζ to phosphorylate transmembrane protein LAT, which facilitates the binding of many signaling proteins. ZAP-70 plays a key role in the activation of T-cells, and their subsequent proliferation, differentiation, and production of cytokines in response to varied stimuli. It is used clinically as a prognostic marker helping differentiate the various types of chronic lymphocytic leukemia (CLL).
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human ZAP 70 peptide phosphorylated at Y493. Complete Freund’s adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: CD3, CD19, and CD56 co-staining indicates that this clone does not bind B-cells and will not bind Syk. Clone A16043B cross-reacts with mouse species. This clone, A16043B, recognizes both non-phosphorylated ZAP-70 and phosphorylated forms of ZAP-70.
Product Citations: Chen HY, et al. 2020. J Immunol. 1158:204. PubMed
RRID: AB_2715832 (BioLegend Cat. No. 693505) AB_2715833 (BioLegend Cat. No. 693506)
Structure: Protein tyrosine kinase, contains two SH2 domains, 70 kD.
Distribution: T cells, NK cells, and B-cell chronic lymphocytic leukemia (B-CLL).
Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Ligand/Receptor: Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc.
Cell Type: Leukemia, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: TCRs
Antigen References: 1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC) 2. Crespo M, et al. 2003. N. Engl. J. Med. 348:1764. (WB, IP) 3. Jamroziak K, et al. 2009. Cancer Epidemiol. Biomarkers Prev. 18:945.
Gene ID: 753522637
UniProt: View information about ZAP-70 on UniProt.org
Clone: A16043B
Regulatory Status: RUO
Other Names: Zeta chain of TCR associated protein kinase 70
Isotype: Mouse IgG1, κ