Biolegend, 695702, Purified anti-Mcl-1 Antibody, 100μg

Myeloid cell leukemia sequence 1 (MCL-1), also known as Bcl2-L-3, is a member of the pro-survival BCL-2 family of apoptotic regulatory proteins. MCL-1 plays a crucial role in the survival and development of various cell types by binding to proapoptotic proteins and inhibiting the mitochondrial apoptotic pathway. Unlike Bcl-2 and Bcl-xL, MCL-1 lacks a BH4 domain and contains an N-terminal PEST region with specific sequences for ubiquitination, phosphorylation, and caspase cleavage sites, which are important in regulating the half-life of MCL-1. Phosphorylation of at T92 and T163 by ERK inhibits MCL-1 turnover, whereas phosphorylation by GSK3β at S159 facilitates proteosomal degradation. Increased expression of MCL-1 is correlated with tumor survival and is associated with resistance of chemotherapeutics.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length mouse MCL-1 recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W16014A does not cross-react with human (in-house tested). This antibody recognizes the two reported isoforms of MCL-1 (MWs: 31 and 35 KDa).
RRID: AB_2650880 (BioLegend Cat. No. 695702)
Structure: There are two reported isoforms of MCL-1 (MWs: 31 and 35 kDa). The protein contains PEST, BH1, BH2, and BH3 domains.
Distribution: Nucleus, Cytoplasm, Mitochondria.
Function: MCL-1 is a prosurvival protein belonging to the BCL-2 family and is required for the survival and development of various cell types.
Interaction: MCL-1 forms a homodimer. Interacts with HIF3A, PMAIP1, BBC3, BAX, BAK1, TPT1, BOP, BCL2L11, BAD, BOK, BIK, and BMF.
Cell Type: Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References: 1. Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA 90:3516. 2. Zhou P, et al. 1997. Blood 89:630. 3. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883. 4. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486. 5. Moulding D, et al. 1998. Blood 92:2495. 6. Inoshita S, et al. 2002. J. Biol. Chem. 277(46):43740. 7. Opferman JT, et al. 2003. Nature. 426:671.
Gene ID: 4170
UniProt: View information about Mcl-1 on UniProt.org
Clone: W16014A
Regulatory Status: RUO
Other Names: Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (BCL2-L-3), Bcl-2-related protein EAT/mcl1,
Isotype: Rat IgG2a, κ