Biolegend, 696602, Purified anti-SARM1 Antibody, 100μg

SARM1 (Sterile alpha and TIR motif-containing protein 1), also known as SAMD2, is a member of the TIR-containing adaptor family. Unlike other TLR adaptor proteins, SARM1 does not activate NF-κB signaling pathway and has been found to be a negative regulator of Toll-Like Receptor-activated transcription. SARM1 is highly expressed in the brain by neurons and has been liked to axonal degeneration following viral infection, axon injury, or oxygen glucose deprivation. Multimerization of SARM1 mediated by the evolutionarily conserved SAM domain is required for its pro-degenerative function. SARM1 also participates in the regulation of neuronal polarity, axonal outgrowth, and brain morphogenesis. Mice deficient in SARM1 demonstrated reduced dendritic arborization and brain size.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial mouse SARM1 recombinant protein (1-410 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Zeng H, et al. 2024. Acta Neuropathol Commun. 23:12. PubMed
RRID: AB_2687069 (BioLegend Cat. No. 696602)
Structure: 724 amino acids with a predicted molecular weight of 79.3 kD. Contains two SAM (sterile alpha motif) domains and a TIR (Toll/interleukin-1 receptor) domain.
Distribution: Cytoplasm. Motochondrion.
Function: SARM1 is an adaptor molecule belonging to the TIR-containing adaptor family. SARM1 is a negative regulator of Toll-Like Receptor-mediated signaling and is required for axonal degeneration and brain morphogenesis.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Summers DW, et al. 2016. Proc. Natl. Acad. Sci. USA. 113:E6271. 2. Loreto A, et al. 2015. Cell. Rep. 13:2539. 3. Mukherjee P, et al. 2015. J. Immunol. 195:4913. 4. Gerdts J, et al. 2015. Science. 348:453. 5. Gilley J, et al. 2015. Cell. Rep. 10:1974. 6. Gerdts J, et al. 2013. J. Neurosci. 33:13569.
Gene ID: 23098
UniProt: View information about SARM1 on UniProt.org
Clone: W16079A
Regulatory Status: RUO
Other Names: Sterile alpha and TIR motif-containing protein 1, Sterile alpha and Armadillo repeat protein, Sterile alpha motif domain-containing protein 2 (SAMD2), MyD88-5, Tir-1 homolog.
Isotype: Rat IgG1, κ