Biolegend, 696904, Alexa Fluor® 594 anti-Prox1 Antibody, 100μg

Prospero homeobox protein 1 (PROX1) is a homeobox transcription factor responsible for progenitor cell differentiation and the development of several organs, such as the central nervous system, lymphatics, pancreas, heart, lens, skeletal muscle, and liver. Prox1-deficient mice lack lymphatic systems and die at day 14.5 of embryogenesis due to multiple developmental defects. In addition to its role in physiological development, PROX1 is involved in carcinogenesis and can act either as a tumor suppressor or as an oncogene depending on the tissue and its expression level. Prox1 is also implicated in the regulation of metabolism in the liver by regulating activities of nuclear receptors including hepatocyte nuclear factor 4a (HNF4a) and liver receptor homolog-1 (LRH-1).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Prox1 recombinant protein (2-259 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 5.0 μg/ml (1:100-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: This clone does not cross react with mouse (in-house tested).
RRID: AB_2734537 (BioLegend Cat. No. 696903) AB_2734538 (BioLegend Cat. No. 696904)
Structure: 737 amino acids with a predicted molecular weight of 83 kD. Contains a C-terminal Prospero-type homeo domain responsible for DNA binding.
Distribution: Nucleus.
Function: PROX-1 is a homeobox transcription factor involved in the embryonic development and carcinogenesis.
Interaction: Interacts with RORA, RORC, and HNF4a.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kivelä R, et al. 2016. Nat. Commun. 7:13124. 2. Stergiopoulos A, et al. 2015. Front Cell Neurosci. 8:454. 3. Ragusa S, et al. 2014. Cell Rep. 8:1957. 4. Elsir T, et al. 2012. Cancer Metastasis Rev. 31:793. 5. Karalay O, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:5807. 6. Johnson NC, et al. 2008. Genes Dev. 22:3282.
Gene ID: 5629
UniProt: View information about Prox1 on UniProt.org
Clone: W16098A
Regulatory Status: RUO
Other Names: Prospero homeobox protein 1, Homeobox prospero-like protein PROX1,
Isotype: Rat IgG2a, κ