Biolegend, 697202, Purified anti-Cytokeratin 17 Antibody, 100μg

Cytokeratin 17 (CK-17), also known as KRT17 or keratin-17 (K-17), is a type I intermediate filament protein. KRT expression is normally restricted to ectoderm-derived epithelial appendages such as thymus, glands, skin, hair, tooth, nail, and the endocervical mucosa. Cytokeratin-17 participates in promoting protein synthesis and cell growth through recruiting stratifin (SFN) to the cytoplasm and stimulating the Akt/mTOR pathway. Cytokeratin-17 was found to regulate chemokine expression, including the CXCR3 ligands CXCL9, CXCL10, and CXCL11. Cytokeratin 17 expression can be upregulated in response to chemical stimulation, wounding, and UV irradiation. Increased expression of Cytokeratin 17 is associated with lesion progression and poor prognosis in epithelial carcinoma. Cytokeratin 17 deficient mice show a delay in wound healing and attenuated tumorigenesis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Cytokeratin 17 peptide (415-432 a.a.) conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 2.0 µg per ml is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not cross-react with mouse (in-house tested). As per the Human Protein Atlas RNA level dataset, A549 cell is expected to have a low level of Cytokeratin 17 expression.
RRID: AB_2687136 (BioLegend Cat. No. 697202)
Structure: 432 amino acids with a predicted molecular weight of 48 kD. Belongs to the type-I intermediate filament family.
Distribution: Cytoplasm
Function: Cytokeratin 17 is a type I intermediate filament protein and is involved in promoting cell growth, chemokine production, and tumorigenesis.
Interaction: Forms a heterodimer with a type I or a type II keratin. Interacts with TRADD and SFN.
Biology Area: Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Hobbs RP, et al. 2016. Oncogene. 35:5653. 2. Escobar-Hoyos LF, et al. 2015. Cancer Res. 75:3650. 3. Chung BM, et al. 2015. J. Cell. Biol. 208:613. 4. Sankar S, et al. 2013. Mol. Cell. Biol. 33:4448. 5. Jin L, et al. 2014. Med. Res. Rev. 34:438. 6. Depianto D, et al. 2010. Nat. Genet. 42:910.
Gene ID: 3872
UniProt: View information about Cytokeratin 17 on UniProt.org
Clone: W16131A
Regulatory Status: RUO
Other Names: Keratin, type I cytoskeletal 17, keratin-17 (K-17), cytokeratin-17 (CK-17), KRT17, CK17, K17
Isotype: Rat IgG2b, κ